| 羔羊STEC的耐药性、毒力基因和血清型分析 |
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| 引用本文： | 张凌,佟盼盼,张毅,马晓玉,张萌萌,刘璐瑶,姚刚,陈童锦悦,苏战强.羔羊STEC的耐药性、毒力基因和血清型分析[J].新疆农业科学,2020,57(10):1921-1930. |
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| 作者姓名： | 张凌 佟盼盼 张毅 马晓玉 张萌萌 刘璐瑶 姚刚 陈童锦悦 苏战强 |
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| 作者单位： | 新疆农业大学动物医学学院,乌鲁木齐 830052 |
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| 基金项目： | 新疆自治区建设专项(自然科学基金)面上项目(2019D01A51);自治区重点研发计划(2017B01005-2-4);新疆农业大学2019年度校级大学生创新项目(S201910758106);国家自然科学基金(31560485) |
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| 摘 要： | 【目的】 研究新疆羊源STEC的耐药性和毒力情况,评价羊源STEC在羊产业链饲养阶段的风险。【方法】 从某规模化羊场随机采取2月龄羊的肛拭子样本,EC肉汤增菌后用MAC平板结合双重PCR(stx1,stx2)法分离鉴定STEC,用SMAC平板结合双重PCR(rfbE和fliC)法分离鉴定E. coli O157:H7;用K-B法做9类18种药的敏感试验,并用PCR检测相应的耐药基因;用PCR检测STEC的毒力基因(eae,ehxA,hlyA,stx1a,stx1c,stx1d,stx2a等),并检测是否“Top Six”和E. coli O157:H7血清型。【结果】 被检的21只羊中,9只携带STEC(42.86%),其中1只羊还携带E. coli O157:H7;药敏试验显示仅有2株菌对头孢吡肟具有耐药性,对其他药均敏感;所有分离菌均携带stx1毒力基因,其毒力基因亚型以stx1a(60%)与stx1c(80%)为主,9株STEC不属于“Top Six”或O157血清型。【结论】 该场羊源STEC的携带率较高,主要携带致病力较弱的stx1,有E. coli O157:H7,但是没有“Top Six”血清群;该场羊源STEC对目前的抗菌素普遍敏感,反映了其良好的养殖环境和较少的抗生素使用。
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| 关 键 词： | :羊 产志贺毒素大肠杆菌 毒力基因亚型 耐药性 血清型 |
| 收稿时间： | 2020-01-13 |
Analysis of Drug Resistance,Virulence Genes and Serotypes of STEC in a Large-scale Sheep Farm in Xinjiang |
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| ZHANG Ling,TONG Pan-pan,ZHANG Yi,Ma Xiao-yu,ZHANG Meng-meng,LIU Lu-yao,YAO Gang,CHEN Tong-jin-yue,SU Zhan-qiang.Analysis of Drug Resistance,Virulence Genes and Serotypes of STEC in a Large-scale Sheep Farm in Xinjiang[J].Xinjiang Agricultural Sciences,2020,57(10):1921-1930. |
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| Authors: | ZHANG Ling TONG Pan-pan ZHANG Yi Ma Xiao-yu ZHANG Meng-meng LIU Lu-yao YAO Gang CHEN Tong-jin-yue SU Zhan-qiang |
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| Institution: | College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China |
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| Abstract: | 【Objective】 To study the drug resistance and virulence of Shiga toxin-producing Escherichia coli from sheep in Xinjing. 【Methods】 Two-month-old sheep's anal swab samples were randomly taken from a large-scale farm. After the samples were enriched in EC broth, the Shiga toxin-producing Escherichia coli was isolated and identified by MAC plate combined with double PCR(stx1, stx2). E. coli O157: H7 was isolated and identified using SMAC plate in combination with double PCR. The susceptibility test of 9 types of 18 drugs was performed using the K-B method. Sensitivity test of 9 types of 18 drugs was conducted by K-B method, and corresponding drug resistance genes were detected by PCR. STEC virulence genes were detected by PCR and tested for "Top Six " and E. coli O157:H7 serotypes. 【Results】 Of the 21 samples, 9 of them contained STEC (42.86%), and one of them contained E. coli O157:H7. Drug susceptibility test showed that only 2 strains were resistant to cefepime and sensitive to other drugs. All isolated strains carry the stx1 virulence gene, and their virulence gene subtypes are mainly stx1a (60%) and stx1c (80%). 9 STECs are not part of the "Top Six" or O157 serotype. 【Conclusion】 From the separation of STEC in the sheep farm, STEC has a high carrying rate, mainly carrying stx1 with weak pathogenicity. The strain isolated from the farm contained the O157:H7 serotype but did not have "Top Six ". The farm's STEC is generally sensitive to current antibiotics, reflecting its good aquaculture environment and less antibiotic use. |
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| Keywords: | sheep Shiga toxin-producing Escherichia coli virulence gene subtype drug resistance serotypes |
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