| 洋葱RAPD遗传多样性分析 |
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| 引用本文： | 崔成日,徐启江,崔崇士,李景鹏.洋葱RAPD遗传多样性分析[J].园艺学报,2006,33(4):863-865. |
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| 作者姓名： | 崔成日 徐启江 崔崇士 李景鹏 |
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| 作者单位： | (东北农业大学园艺学院, 黑龙江哈尔滨150030; 东北林业大学生命科学学院, 黑龙江哈尔滨150040; 东北农业大学生命科学学院, 黑龙江哈尔滨150030) |
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| 基金项目： | 哈尔滨市科技局重点科技攻关项目(2003AA6BN060) |
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| 摘 要： | 利用RAPD技术对41份洋葱种质资源的遗传多样性进行了分析。从100个随机引物中筛选出7个引物, 共扩增出64个位点, 其中多态性位点34个(53113% ) 。材料间遗传相似系数变化范围为0.694～1.000。在遗传相似系数为0.926水平上, 聚类分析结果可将供试材料分为9类, 聚类结果与形态学特征有一定对应关系。
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| 关 键 词： | 洋葱 RAPD 遗传多样性 聚类分析 |
| 文章编号： | 0513-353X（2006）04-0863-03 |
| 收稿时间： | 2005-10-09 |
| 修稿时间： | 2005-10-092006-04-04 |
An Analysis of Onion Genetic Diversity by RAPD |
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| Cui Chengri,Xu Qijiang,Cui Chongshi,Li Jingpeng.An Analysis of Onion Genetic Diversity by RAPD[J].Acta Horticulturae Sinica,2006,33(4):863-865. |
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| Authors: | Cui Chengri Xu Qijiang Cui Chongshi Li Jingpeng |
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| Institution: | (Colllege of Horticulture, Northeast Agricultural University, Harbin, Heilongjiang 150030, China; Colllege of Life Science, Northeast Forestry University, Harbin, Heilongjiang 150040, China; Colllege of Life Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China) |
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| Abstract: | The genetic diversity of 41 onion (Allium cepa L.) germplasm resources were analyzed by RAPD(Random Amplified Polymorphic DNA). Results showed that 34 (53.13%) polymorphic sites were detected among the total 64 sites amplified by 7 screened random primers (among 100 primers). The RAPD-based genetic similarity ranged from 0.694 to 1.000. UPGMA cluster analysis showed that the tested materials could be divided into nine groups at the level of genetic similarity 0.926. Dendrogram obtained using molecular markers is homologous with separated morphologic character in some degree. |
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| Keywords: | Onion RAPD Genetic diversity Cluster analysis |
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