| 表达H5亚型禽流感病毒HA基因的重组鸡痘病毒在水禽的免疫效力 |
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| 引用本文： | 贾立军,刘秀梵,张艳梅,彭大新,张如宽.表达H5亚型禽流感病毒HA基因的重组鸡痘病毒在水禽的免疫效力[J].中国兽医学报,2004,24(5):417-419. |
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| 作者姓名： | 贾立军 刘秀梵 张艳梅 彭大新 张如宽 |
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| 作者单位： | 扬州大学,畜牧兽医学院,江苏,扬州,225009 |
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| 基金项目： | 国家高新技术研究发展计划项目 (86 3-2 0 0 1 AA2 1 30 41 )，农业部农办牧项目 (2 0 0 1 -83) |
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| 摘 要： | 利用表达 H5亚型禽流感病毒血凝素基因的重组鸡痘病毒 ( r FPV- HA)和油乳剂全病毒灭活疫苗分别接种商品鹅 ,评价疫苗在水禽的免疫保护作用。结果发现 ,免疫后 2 1 d,r FPV- HA免疫组 HI抗体检测为阴性 ,灭活疫苗免疫组HI抗体阳性率为 70 % ;用 H5亚型禽流感病毒攻击后 ,与阴性对照组相比 ,r FPV - HA免疫组鹅的口腔和泄殖腔排毒率降低 ,病理变化减轻 ,感染鹅易康复 ,保护率为 80 % ,总体保护效力达到灭活疫苗水平。结果表明 r FPV - HA免疫家鹅可诱导良好保护 ,显示出了良好的应用前景
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| 关 键 词： | H5亚型 禽流感病毒 重组鸡痘病毒 水禽 免疫效力 |
| 文章编号： | 1005-4545(2004)05-0417-02 |
| 修稿时间： | 2003年7月15日 |
Protective Efficacy of Recombinant Fowlpox Virus-HA Vaccine against H5 Subtype of Avian Influenza Virus in Domestic Geese |
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| JIA Li-jun,LIU Xiu-fan,ZHANG Yan-mei,PENG Da-xin,ZHANG Ru-kuan.Protective Efficacy of Recombinant Fowlpox Virus-HA Vaccine against H5 Subtype of Avian Influenza Virus in Domestic Geese[J].Chinese Journal of Veterinary Science,2004,24(5):417-419. |
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| Authors: | JIA Li-jun LIU Xiu-fan ZHANG Yan-mei PENG Da-xin ZHANG Ru-kuan |
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| Institution: | JIA Li-jun,LIU Xiu-fan~*,ZHANG Yan-mei,PENG Da-xin,ZHANG Ru-kuan |
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| Abstract: | Groups of 21-day-old geese were inoculated subcutaneously with recombinant fowlpox virus expressing the hemagglutinin(HA) gene of H5 subtype avian influenza virus(rFPV-HA),inactivated vaccine and wild type fowlpox virus,respectively.Vaccinated geese were challenged with highly pathogenic avian influenza virus of H5 subtype by oral,intranasal and intramuscular inoculation,21 days after immunization.No geese vaccinated with rFPV-HA developed HI antibody post immunization.While 70% geese immunized with inactivated vaccine produced HI antibody.But both rFPV-HA and inactivated vaccine protected 80% geese from death,reduced virus shedding and shortened the period of infection after challenge.The general protective efficacy of rFPV-HA was equal to the inactivated vaccine. |
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| Keywords: | H5 subtype avian influenza virus recombinant fowlpox virus goose protective efficacy |
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