| 广西北海红树林土壤放线菌BH0954的分离和鉴定 |
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| 引用本文： | 黄大林,袁桂峰,徐雅娟,骆耐香,陈建宏,刘菁,蒋莲秀,陈森洲.广西北海红树林土壤放线菌BH0954的分离和鉴定[J].中国农学通报,2010,26(18):406-409. |
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| 作者姓名： | 黄大林 袁桂峰 徐雅娟 骆耐香 陈建宏 刘菁 蒋莲秀 陈森洲 |
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| 作者单位： | 桂林医学院,广西桂林,541004 |
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| 基金项目： | 广西教育厅科研资助项目"广西红树林放线菌的多样性研究" |
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| 摘 要： | 摘要：【目的】对具有抗菌活性红树林土壤放线菌的进行分离和鉴定。【方法】用海水配制高氏一号培养基，采用稀释分离法将所采集的土壤样本进行分离纯化，通过抗菌试验筛选，从广西北海红树林高盐泥样中分离到一株放线菌BH0954，该菌株的发酵产物具有很强的抗菌活性。该菌株在高氏一号培养基上生长良好，最适生长温度28 ℃，最适生长pH为7.0，观察其形态特征和生理生化特征；提取其基因组总DNA，用通用引物对其16s rDNA进行PCR扩增，扩增产物进行DNA序列测定。【结果】根据其形态学特征、生理生化特征和基于16S rDNA基因序列的系统发育分析结果，BH0954初步被鉴定为链霉菌属的有效发表种仙台链霉菌（Streptomyces sindenensis）的变种。【结论】红树林土壤放线菌BH0954具有抗菌活性，本文的研究结果为进一步开发利用广西红树林土壤放线菌资源奠定基础。
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| 关 键 词： | HIVs HIVs 释放机制 应用 |
| 收稿时间： | 2010/3/29 0:00:00 |
| 修稿时间： | 2010/5/17 0:00:00 |
Beihai, Guangxi Mangrove soil actinomycetes BH0954 Isolation and identification of |
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| Huang Dalin,Yuan Guifeng,Xu Yajuan,Luo Naixiang,Chen Jianhong,Liu Jing,Jiang lianxiu,Chen Senzhou.Beihai, Guangxi Mangrove soil actinomycetes BH0954 Isolation and identification of[J].Chinese Agricultural Science Bulletin,2010,26(18):406-409. |
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| Authors: | Huang Dalin Yuan Guifeng Xu Yajuan Luo Naixiang Chen Jianhong Liu Jing Jiang lianxiu Chen Senzhou |
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| Institution: | (Guilin Medical University, Guilin Guangxi 541004) |
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| Abstract: | Objective]Isolation and identification ofActinomycetes sp of the activity of antibacteria from mangrove soil.Methods]Based on the morphological characteristics, physiological and biochemical characteristics and 16S rDNA sequence homology analysis, an isolated bacterium from the samples of salt-high soil from Beihai mangrove forest was identifed.Results]Phylogenetic analysis of the strain based on comparison of 16S rRNA sequence revealed that it was closely related toStreptomyces. sindenensis with 99% identity. So, the isolated strain was identified as one species ofS. sindenensis. Conclusion]It showed that Actinomyces sp. BH0954 was of antibacterial activity. And thus would provide the original data for developing and exploiting the strain of BH0954 and the other Actinomyces resources of the mangrove forest soil of Guangxi Beihai. |
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| Keywords: | 16S rDNA |
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