| 玉米大斑病菌黑色素合成酶基因4HNR的克隆及其启动子预测 |
| |
| 引用本文： | 温雷蕾,曹志艳,董金皋.玉米大斑病菌黑色素合成酶基因4HNR的克隆及其启动子预测[J].植物病理学报,2008,38(6):641-644. |
| |
| 作者姓名： | 温雷蕾 曹志艳 董金皋 |
| |
| 作者单位： | 河北农业大学植物保护学院植物分子病理学实验室, 保定 071001 |
| |
| 基金项目： | 国家自然科学基金，河北省自然科学基金 |
| |
| 摘 要： | 4HNR (1,3,6,8-tetra-HN reductase) gene of melanin biosynthesis in Setosphaeria turcica had been cloned successfully by RT-PCR in this study. Both sequences of DNA and cDNA of 4HNR were 807 bp and there was no intron in the sequences. This gene only had single copy in genome through Southern blotting analysis. A 2 285 bp for the flanking sequence of 5' had been obtained and it had promoter structure through the software analysis.
|
| 关 键 词： | 玉米大斑病菌 黑色素 合成酶基因 启动子 预测 克隆 机械压力 附着胞 |
Cloning and promoter prediction of melanin biosynthesis gene 4HNR in Setosphae-ria turcica |
| |
| WEN Lei-lei,CAO Zhi-yan,DONG Jin-gao.Cloning and promoter prediction of melanin biosynthesis gene 4HNR in Setosphae-ria turcica[J].Acta Phytopathologica Sinica,2008,38(6):641-644. |
| |
| Authors: | WEN Lei-lei CAO Zhi-yan DONG Jin-gao |
| |
| Institution: | Molecular Plant Pathology Lab., College of Plant Protection, Agricultural University of Hebei, Baoding 071001, China |
| |
| Abstract: | 4HNR(1,3,6,8-tetra-HN reductase)gene of melanin biosynthesis in Setosphaeria turcica had been cloned successfully by RT-PCR in this study.Both sequences of DNA and cDNA of 4HNR were 807 bp and there was no intron in the sequences.This gene only had single copy in genome through Southern blotting analysis.A 2 285 bp for the flanking sequence of 5' had been obtained and it had promoter structure through the software analysis. |
| |
| Keywords: | Setosphaeria turcica melanin 4HNR promoter |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《植物病理学报》浏览原始摘要信息 |
| 点击此处可从《植物病理学报》下载免费的PDF全文 |
