| 瓜黑星病菌、枯萎病菌和蔓枯病菌的三重PCR检测 |
| |
| 引用本文： | 高永洋,王楠,高观朋,王伟.瓜黑星病菌、枯萎病菌和蔓枯病菌的三重PCR检测[J].植物病理学报,2010,40(4):343-350. |
| |
| 作者姓名： | 高永洋 王楠 高观朋 王伟 |
| |
| 作者单位： | 华东理工大学 生物反应器工程国家重点实验室, 上海 200237 |
| |
| 基金项目： | 国家自然科学基金项目，上海市重点攻关项目，国家重点实验室专项经费 |
| |
| 摘 要： | 通过测定黄瓜黑星病菌(Cladosporium cucumerinum)rDNA的ITS序列,比对近缘种及瓜类几种重要病原菌的ITS序列,设计出特异性引物HX-1/HX-2,经过对引物HX-1/HX-2PCR条件的优化,可以扩增出1条190bp的黄瓜黑星病菌特异性DNA条带,灵敏度达到1pg/μL。进一步将引物HX-1/HX-2和瓜类枯萎病菌、瓜类蔓枯病菌特异检测引物Fn-1/Fn-2、Mn-1/Mn-2组合,建立三重PCR体系,可一次检测出瓜类黑星病菌、瓜类枯萎病菌、瓜类蔓枯病菌3种瓜类植物重要的病原菌。建立了可以应用于田间瓜类黑星病菌PCR检测技术和瓜类主要病害三重PCR检测技术,对瓜类病害的诊断和防治具有重要的指导作用。
|
| 关 键 词： | 瓜黑星病菌 瓜枯萎病菌 瓜蔓枯病菌 分子检测 三重PCR |
Triplex PCR detection of Cladosporium cucumerinum,Fusarium oxysporum f.sp.niveum and Mycosphaerella melonis in infected plant tissues |
| |
| GAO Yong-yang,WANG Nan,GAO Guan-peng,WANG Wei.Triplex PCR detection of Cladosporium cucumerinum,Fusarium oxysporum f.sp.niveum and Mycosphaerella melonis in infected plant tissues[J].Acta Phytopathologica Sinica,2010,40(4):343-350. |
| |
| Authors: | GAO Yong-yang WANG Nan GAO Guan-peng WANG Wei |
| |
| Institution: | State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China |
| |
| Abstract: | rDNA ITS sequences of Cladosporium cucumerinum were sequenced. A pair of specific primers HX-1/HX-2 was designed from homology comparison of ITS sequences of C. cucumerinum with closely-rela-ted species and some pathogenic fungi on cucurbitaceous plants. A single special PCR band of 190 bp existed in C. cucumerinum was amplified under the optimized PCR parameters using HX-1/HX-2 primers and the detection sensitivity was 1 pg/μL of genomic DNA. Then a multiplex PCR system was developed to detect C. cucumerinum, Fusarium oxysporum f. sp. niveum and Mycosphaerella melonis simultaneously with combining primers HX-1/HX-2, Fn-1/Fn-2 and Mn-1/Mn-2. It was the first time to establish the triplex PCR system for detecting C. cucumerinum, F. oxysporum f. sp. niveum and M. melonis in infected plant tissues simultaneously. The PCR-based method developed here could direct the diagnosis of plant disease and pathogen monitoring. |
| |
| Keywords: | Cladosporium cucumerinum Fusarium oxysporum f sp niveum Mycosphaerella melonis molecular detection triplex PCR |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《植物病理学报》浏览原始摘要信息 |
| 点击此处可从《植物病理学报》下载免费的PDF全文 |
|
