| 甘肃省西瓜细菌性果斑病的诊断 |
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| 引用本文： | 薛莉,杨成德,陈秀蓉,贾迎春,雒东林.甘肃省西瓜细菌性果斑病的诊断[J].植物保护,2009,35(2):53-57. |
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| 作者姓名： | 薛莉 杨成德 陈秀蓉 贾迎春 雒东林 |
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| 作者单位： | 1. 甘肃农业大学草业学院,草业生态系统教育部重点实验室
2. 甘肃省植保植检站,兰州,730020 |
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| 摘 要： | 对从甘肃采集到的疑似西瓜果斑病的病瓜及种子中分离获得的菌株，通过形态学特征、革兰氏染色、致病性测定以及利用美国Agdia公司的专化型免疫凝聚试剂条测定和16S rDNA分子生物学方法进行了诊断鉴定，结果表明,该菌株符合燕麦噬酸菌西瓜亚种(Acidovorax avenae subsp. citrulli)的特性，因此，确定甘肃省已发生西瓜果斑病，结果为甘肃省有效控制此病提供了依据。
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| 关 键 词： | 西瓜细菌性果斑病 致病性测定 形态特征 16S rDNA鉴定 |
Identification of the bacterial blotch of watermelon in Jiuquan district of Gansu Province |
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| Xue Li,Yang Chengde,Chen Xiurong,Jia Yingchun,Luo Donglin.Identification of the bacterial blotch of watermelon in Jiuquan district of Gansu Province[J].Plant Protection,2009,35(2):53-57. |
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| Authors: | Xue Li Yang Chengde Chen Xiurong Jia Yingchun Luo Donglin |
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| Institution: | 1. Key Laboratory of Grassland Ecosystem, Sino US Center for Grazingland Ecosystem Sustainability, College of Grasslands, Gansu Agricultural University, Lanzhou730070, China;2. Plant Protection and Quarantine Station of Gansu Province, Lanzhou730020, China |
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| Abstract: | Some bacterial samples were isolated and purified from watermelon and its seeds in Gansu. The pathogenicity of the bacteria with G was detected. The results showed that only one isolate could cause typical symptoms of Acidovorax avenae subsp. citrulli. The strain was detected by special variety immuno strip and the results was positive. Furthermore, the strain was identified as Acidovorax avenae subsp. citrulli by morphological characteristics and 16S rDNA sequence, which helped control this disease in Gansu. |
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| Keywords: | bacterial blotch of watermelon pathogenicity test morphological characteristics 16S rDNA identification |
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