| 精子介导法制备转基因鸡的研究 |
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| 引用本文： | 苗向阳,丁淑燕,郝慧芳,杨文胜.精子介导法制备转基因鸡的研究[J].中国畜牧兽医,2007,34(7):67-69. |
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| 作者姓名： | 苗向阳 丁淑燕 郝慧芳 杨文胜 |
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| 作者单位： | 中国农业科学院北京畜牧兽医研究所，北京 100094 |
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| 基金项目： | 中国农业科学院院长基金;中国农业科学院资助项目 |
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| 摘 要： | 以LacZ基因为报告基因，构建载体质粒p-OV-β-Gal，转移到鸡早期胚胎内，对外源基因的整合及表达情况进行检测分析，为制备转基因鸡探索新的方法。采用脂质体介导法、精子介导法以及电激法相结合的方法将载体质粒p-OV-β-Gal转移到鸡早期胚胎内，进行转基因鸡研究，结果发现其外源基因整合阳性率为13.2%（7/53），表达阳性率为20.7%（11/53）。说明该方法可作为制备转基因鸡的有效方法。
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| 关 键 词： | 鸡 转基因 精子介导法 |
| 文章编号： | 1671-7236（2007）07-0067-02 |
| 修稿时间： | 2006-12-21 |
Production of Transgenic Chicken by Sperm-mediated Method |
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| MIAO Xiang-yang,DING Shu-yan,HAO Hui-fang,YANG Wen-sheng.Production of Transgenic Chicken by Sperm-mediated Method[J].China Animal Husbandry & Veterinary Medicine,2007,34(7):67-69. |
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| Authors: | MIAO Xiang-yang DING Shu-yan HAO Hui-fang YANG Wen-sheng |
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| Institution: | 1.Institute of Animal Sciences, Chinese Academy of Agriculture Sciences, Beijing 100094, China; 2.College of Animal Science and Technology, Shandong Agricultural University, Taian 271018, China |
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| Abstract: | To explore the possibility of generation of transgenic chicken, vector p-OV-β-Gal of LacZ gene as report gene were transmitted into the chicken''s early embryos by sperm-mediated method, then the LacZ gene’s integration and expression were monitored. The vector (p-OV-β-Gal) could be transmitted into the chicken’s early embryos by sperm-mediated method, the percentage of LacZ gene''s integration and expression were respectively 13.2%（7/53）and 20.7%（11/53）. The result showed that sperm-mediated method could be used for generation of the transgenic chickens. |
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| Keywords: | chicken transgene sperm-mediated method |
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