|
|||||
|
|
| 不同地域来源的家蚕微孢子虫DNA特异片段序列及同源性分析 | |
| 引用本文： | 时连辉,邱宝利,高绘菊.不同地域来源的家蚕微孢子虫DNA特异片段序列及同源性分析[J].蚕业科学,2003,29(4):384-386. |
| 作者姓名： | 时连辉 邱宝利 高绘菊 |
| 作者单位： | 1. 山东农业大学科技学院,泰安,271018;山东农业大学林学院 2. 华南农业大学昆虫学系 3. 山东农业大学科技学院,泰安,271018 |
| 摘 要： | 利用碱裂解方法抽提到山东株系的家蚕微孢子虫 (Nosemabombycis,N .b)的基因组DNA ,通过PCR技术扩增得到了N .b的一段特异的DNA片段 ,经测序发现 ,该片段大小为 317bp。将其与日本株N .b和广东株N .b的序列比较得知 ,山东株N .b和广东株N .b ,山东株N .b和日本株N .b ,以及广东株N .b和日本株N .b之间的序列同源性分别为 94 .0 0 %、96 .2 1%、92 .11%。进一步聚类分析发现 ,山东株N .b和日本株N .b之间的亲缘关系更接近 |
| 关 键 词： | 家蚕微孢子虫 DNA特异性片段 序列分析 同源性 |
| 文章编号： | 0257-4799(2003)04-0384-03 |
| 修稿时间： | 2003年4月21日 |
Study on the Comparision of Sequence Similarity of Three Nosema bombycis Geographical Strains |
|
| SHI Lianhui , QIU Baoli GAO Huiju.Study on the Comparision of Sequence Similarity of Three Nosema bombycis Geographical Strains[J].Acta Sericologica Sinica,2003,29(4):384-386. | |
| Authors: | SHI Lianhui QIU Baoli GAO Huiju |
| Institution: | SHI Lianhui 1,2 QIU Baoli 3 GAO Huiju 2 |
| Abstract: | The genome DNA of Nosema bombycis (N. b) from Shandong Province was extracted using SDS and proteinase K. A specific DNA fragment was synthesized by PCR amplification and the genome DNA of N.b was used as the template. The result of sequencing showed that the DNA fragment was 317 bp. The identity between Shandong N.b and Japanese N.b, Shandong N.b and Guangdong N.b, Guangdong N.b and Japanese N.b were 96.21%, 94.00% and 92.11% respectively. The result of cluster showed that Shandong N.b was more closed to Japanese N.b than to Guangdong N.b. |
| Keywords: | Nosema bombycisDNA specific fragmentSequence analysisIdentity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |