| 1个油茶Y2SK2型脱水素的全长cDNA克隆及生理功能的预测 |
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| 引用本文： | 胡孝义,谭晓风,张党权,曾艳铃,陈鸿鹏,田晓明,刘巧.1个油茶Y2SK2型脱水素的全长cDNA克隆及生理功能的预测[J].林业科学,2008,44(10). |
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| 作者姓名： | 胡孝义 谭晓风 张党权 曾艳铃 陈鸿鹏 田晓明 刘巧 |
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| 作者单位： | 中南林业科技大学经济林育种与栽培国家林业局重点实验室,长沙,410004 |
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| 基金项目： | 国家科技支撑项目，国家自然科学基金，湖南省科技厅科研项目 |
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| 摘 要： | 以构建的油茶EST文库为基础,采用电子克隆技术,分离克隆一个脱水素基因的全长cDNA序列,该基因的cDNA全长1 406 bp,含一个600 bp的CDS,编码200明的小分子蛋白.推测该基因编码蛋白的分子质量为21 ku,等电点7,暂命名为CoDHNI.同源性分析发现该基因的编码蛋白具有2个Y-片段(DEYGNP),1个S-片段(SGSSSSSS),2个K-片段(KIKEKLPG),属于典型的Y2sIQ型脱水素.经Blast比对,发现富含苏氨酸的Thr-区在各物种间变异显著,推测该区为油茶所特有,有利于在被保护的大分子表面形成水合层;同时还发现一个十分保守的基序:EDDGQGGRRKK,可能有利于脱水素的磷酸化和亚细胞定位.通过与柑桔和拟南芥的脱水素比较,提出CoDHNI有可能缓冲种子脱水胁迫响应时钙离子的瞬时增高,并可结合重金属离子,以减轻活性氧的危害.
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| 关 键 词： | 油茶 脱水素基因 cDNA文库 生理功能 生物信息学 |
A Full-Length cDNA Clone of Typical Y2SK2 Dehydrin and Its Speculated Physiological Role in the Seeds of Camellia oleifera |
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| Hu Xiaoyi,Tan Xiaofeng,Zhang Dangquan,Zeng Yanling,Chen Hongpeng,Tian Xiaoming,Liu Qiao.A Full-Length cDNA Clone of Typical Y2SK2 Dehydrin and Its Speculated Physiological Role in the Seeds of Camellia oleifera[J].Scientia Silvae Sinicae,2008,44(10). |
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| Authors: | Hu Xiaoyi Tan Xiaofeng Zhang Dangquan Zeng Yanling Chen Hongpeng Tian Xiaoming Liu Qiao |
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| Institution: | Key Laboratory of Non-Wood Forest Products of State Forestry Administratrion Central South University of Forestry and Technology Changsha 410004 |
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| Abstract: | Based on a constructed EST library of Camellia oleifera,a full-length cDNA encoding a dehydrin was cloned from its seed by the electronic PCR technique. The cDNA,tentatively designated as CoDHN1,was 1 406 bp long containing an open reading frame of 600 bp. The deduced CoDHN1 protein had 200 amino acid residues,which formed a 21 ku polypeptide with a predicted isoelectric point (pI) of 7. The CoDHN1 protein had two Y-segments,an S-segment and two K-segments. Homology analysis indicated that the Y-segment,S-segment and K-segment of CoDHN1 shared identity with those of other reported dehydrins,indicating that CoDHN belonged to dehydrin superfamily. There was a wide range of variation for Thr-region existed among dehydrins from various species,and the CoDHN1 Thr-region was Camellia oleifera specific,protecting some macromolecules by forming hydrated layer outside of them. A very conserved motif of EDDGQGGRRKK was found in CoDHN1,and it might contribute to dehydrin's phosphorylation and subcellular localization. By comparison with dehydrins from citrus and Arabidopsis respectively,it was proposed that CoDHN1 might serve as a buffer for transiently increased Ca2 when the matured seeds was under the condition of desiccation stress to some extent,and that CoDHN1 might bind heavy metal ions such as Cu2 ,Ni2 ,Zn2 ,Co2 ,Fe3 and hence scavenge reactive oxygen species to alleviate toxin caused by them. |
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| Keywords: | RACE |
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