| 斑唇马先蒿挥发油的超临界萃取及GC-MS分析 |
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| 引用本文： | 王劼,陈保业,晁玉龙,邓娟,孙菁,袁园园,周玉碧.斑唇马先蒿挥发油的超临界萃取及GC-MS分析[J].甘肃农业大学学报,2017,52(2). |
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| 作者姓名： | 王劼 陈保业 晁玉龙 邓娟 孙菁 袁园园 周玉碧 |
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| 作者单位： | 1. 中国科学院藏药研究重点实验室,青海省青藏高原特色生物资源研究重点实验室,中国科学院 西北高原生物研究所,青海 西宁810008;中国科学院大学,北京 100049;2. 祁连县林业站,青海 祁连,810499;3. 中国科学院藏药研究重点实验室,青海省青藏高原特色生物资源研究重点实验室,中国科学院 西北高原生物研究所,青海 西宁810008 |
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| 基金项目： | 青海省科技计划项目，青海省基础研究计划项目，国家自然科学基金青年基金项目 |
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| 摘 要： | 【目的】探究斑唇马先蒿挥发油的最优超临界萃取条件及其化学组成.【方法】通过正交试验筛选斑唇马先蒿挥发油的最优萃取条件.将最优萃取条件下得到的挥发油,经柱色层族组成分离为非极性、弱极性和极性3种馏分,并进行了GC-MS分析.【结果】斑唇马先蒿挥发油最优萃取条件为:压力25 Mpa,温度45℃,CO2流量16mL/min.斑唇马先蒿挥发油中共鉴定出127种化合物,其中非极性馏分、弱极性馏分、极性馏分的主要组成成分分别为烷烃类、酯类和脂肪酸类.在其挥发油成分中,脂肪酸类化合物占绝对优势,总相对含量为62.698%;其中亚麻酸(18.497%)的相对含量最高,其次为正十六烷酸(15.698%)和亚油酸(7.286%).【结论】本研究获得的斑唇马先蒿挥发油的最优萃取条件温和,效率高,其挥发油的化学成分种类丰富.
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| 关 键 词： | 斑唇马先蒿 挥发油 超临界萃取 GC-MS |
GC-MS analysis of essential oil from Pedicularis iongiflora var.tubiformis by supercritical fluid extraction |
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| Abstract: | Objective] To analyze the chemical compositions of essential oil from Pedicularis longiflora var.Tubiformi,by supercritical fluid extraction.Method] The extraction conditions of the essential oil with supercritical fluid extraction were optimized by orthogonal experiment.The essential oil was separated into three parts as non-polar,weak polar and polar fractions by column chromatography.Result] A total of 127 compounds of the essential oil were identified by GC-MS.The fatty acids,esters and alkane were major grouped components of the three fractions,respectively.Fatty acids were the most dominate components and their relative content reached 62.698%.9,12,15-octadecatrienoic acid (18.497%),hexadecanoic acid (15.698%) and 9,12-octadecadienoic acid (7.286%) were dominant compounds.Conclusion] Extraction conditions of the essential oil with supercritical fluid extraction were milder,and the kinds of compositions of the essential oil are abundant. |
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| Keywords: | Pedicularis longiflora var tubiformis essential oil supercritical fluid extraction GC-MS |
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