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| 板栗优良品种（无性系）苗木分子标记鉴别研究） | |
| 引用本文： | 翁尧富,陈源,郑康乐,赵勇春,黄少甫.板栗优良品种（无性系）苗木分子标记鉴别研究）[J].林业科学,2001,37(2):51-55. |
| 作者姓名： | 翁尧富 陈源 郑康乐 赵勇春 黄少甫 |
| 作者单位： | 1. 浙江省林业厅林木种苗站 2. 中国水稻研究所 3. 中国林业科学研究院亚热带林业研究所 |
| 基金项目： | 浙江省科委重点攻关课题 项目编号!96 110 2 10 8 |
| 摘 要： | 利用ＲＡＰＤ标记对浙江省林木良种审定委员会审定（认定）和通过省级鉴定的８个板栗品种及无性系进行指纹分析，鉴别出各板栗品种（无性系），实验过程中对６８４个随机核苷酸引物进行了重复筛选，经筛选共获得９个稳定的ＲＡＰＤ标记，构建了板栗的ＮＤＡ指纹图谱，经过大量的实验，确定了鉴别板栗优良品种（无性系）的较为理想的程序，获得了ＲＡＰＤ良好的重复性，建立了较完整的板栗ＲＡＰＤ分析的技术体系，用大田苗进行步验证，结果可靠。 |
| 关 键 词： | 板栗 品种 无性系 鉴别 DNA指纹图谱 PAPD标记 |
| 修稿时间： | 2000年2月14日 |
DNA FINGERPRINTING OF CASTANEA MOLLISSIMA ELITE VARIETY (CLONE) SEEDLINGS |
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| Weng Yaofu,Chen Yuan,Zheng Kangle,Zhao Yongchun,Huang Shaofu.DNA FINGERPRINTING OF CASTANEA MOLLISSIMA ELITE VARIETY (CLONE) SEEDLINGS[J].Scientia Silvae Sinicae,2001,37(2):51-55. | |
| Authors: | Weng Yaofu Chen Yuan Zheng Kangle Zhao Yongchun Huang Shaofu |
| Institution: | Weng Yaofu 1) \ Chen Yuan 2) \ Zhao Yongchun 4) (Seed and Seedling Station of Zhejiang Forestry Department\ Hangzhou 310020) Zheng Kangle 3) (China National Rice Research Institute\ Hangzhou 310006) Huang Shaofu 5) (Subtropical For |
| Abstract: | RAPD\_\_based fingerprinting was carried out for 8 varieties and clones of \%Castanea mollissima\% which had been approved by the Zhejiang Inspection Committee of Superior Tree Species.Each of the varieties (clones) was identified by fingerprints.Nine RAPD markers were obtained from repeated screening of 684 random oligonucleotide primers and DNA fingerprints were made for all the used varieties (clones).Stable amplification was obtained through modification of experimental conditions and a complete technical system was established for fingerprinting of \%Castanea mollissima.\%Preliminary results for verification of commericial seedilings showed that these fingerprints were reliable. |
| Keywords: | Castanea mollissima \%Variety (clone) identification Random amplified polymorphismic DNA DNA fingerprinting |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |