| 结球白菜带柄子叶外植体不定芽离体再生能力的遗传分析 |
| |
| 引用本文： | 杨慧莹,王鸣刚,武丽娜,张凤兰,刘凡.结球白菜带柄子叶外植体不定芽离体再生能力的遗传分析[J].广西农业生物科学,2010(2):332-338. |
| |
| 作者姓名： | 杨慧莹 王鸣刚 武丽娜 张凤兰 刘凡 |
| |
| 作者单位： | [1]兰州理工大学生命科学与工程学院,兰州730050 [2]北京市农林科学院蔬菜研究中心,北京100097 |
| |
| 基金项目： | 本研究由国家863项目（2008AA102154）和北京市科技合同项目（Z07070501770704）共同资助 |
| |
| 摘 要： | 解析控制植物不定芽离体再生能力的遗传因素，有助于从根本上提高离体培养困难材料的不定芽再生能力，是植物基因工程遗传改良的基础。本研究以结球白菜4个纯合亲本及其杂交后代的带柄子叶为外植体，建立了高频率离体不定芽再生技术。据此我们还对白菜不定芽再生能力进行遗传分析，并开展亲本与后代杂种的离体培养及反应研究，包括对培养基的激素需求，不定芽形成频率，不定芽形成数量，芽形态等系列特征。研究结果表明，杂种与亲本在培养基的激素浓度上有类似需求；杂交后代的不定芽再生率均至少高于一方亲本，在一些组合中可以高于双亲；在适宜的杂交组合及培养条件下，结球白菜带柄子叶外植体的不定芽再生率可以达100％，每一外植体上的不定芽数达3～7个。方差分析表明，结球白菜的不定芽离体再生能力存在基因的加性效应。
|
| 关 键 词： | 结球白菜 外植体 不定芽再生 遗传分析 |
Genetic Analysis of in vitro Adventitious Shoot Regeneration from Cotyledonary Petioles Explant in Chinese Cabbage |
| |
| Yang Huiying,Wang Minggang,Wu Lina,Zhang Fenglan,Liu Fan.Genetic Analysis of in vitro Adventitious Shoot Regeneration from Cotyledonary Petioles Explant in Chinese Cabbage[J].Journal of Guangxi Agricultural and Biological Science,2010(2):332-338. |
| |
| Authors: | Yang Huiying Wang Minggang Wu Lina Zhang Fenglan Liu Fan |
| |
| Institution: | Yang Huiying Wang Minggang Wu Lina Zhang Fenglan Liu Fan (1 College of Life Sciences and Technology, Lanzhou University of Technology, Lanzhou, 730050; 2 Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097) |
| |
| Abstract: | Resolving the genetic factors of controlling plant adventitious shoot regeneration in vitro can improve the regeneration frequency of some recalcitrate species radically, which is the base of plant genetic engineering. In this research, we employed the cotyledonary petioles in Chinese cabbage of four parental lines and their crossed hybrids as explant, and established the techniques with a high shoot regeneration frequency in vitro. Based on these, we still studied the genetic analysis on the regenerating ability of Chinese cabbage and carried on the invastigation of in vitro cultivation and response of parental lines and their crossed hybrids, including the series of characteristics of requirement on the hormone concentration, the shoot regeneration frequency, the shoot morphology and shoot number per explant etc. The results demonstrated that there was a parallelism requirement on the hormone concentration in the medium between the parents and their hybrids; the shoot regeneration frequency of the hybrid was at least higher than one of the parents, and it was even obviously higher than both of the parents in some cross combinations. Furthermore, during the fitting cross combination and culture condition, the highest regeneration frequency of the cotyledonary petioles in Chinese cabbage could reach 100% and obtained 3-7 shoots per explant. Analysis of variance showed that the occurrence of additive gene effects in the in vitro shoot regeneration in Chinese cabbage. |
| |
| Keywords: | Chinese cabbage （Brassiea campestris L ssp pekinensis） Explant Shoot regeneration Genetic analysis |
| 本文献已被 维普 等数据库收录！ |
|
