| 猪细小病毒自然弱毒N株VP2基因的克隆、测序及生物信息学分析 |
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| 引用本文： | 李斌,梁家幸,赵武,苏乾莲,何颖,梁保忠,秦毅斌,何苹萍.猪细小病毒自然弱毒N株VP2基因的克隆、测序及生物信息学分析[J].广西农业生物科学,2010(5). |
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| 作者姓名： | 李斌 梁家幸 赵武 苏乾莲 何颖 梁保忠 秦毅斌 何苹萍 |
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| 作者单位： | 广西兽医研究所 |
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| 基金项目： | 广西自然科学基金项目(桂科自0728102);; 广西基本科研业务费专项(桂兽研专项09-4)共同资助 |
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| 摘 要： | 本研究对猪细小病毒(porcine parvovirus,PPV)自然弱毒N株(PPV-N)VP2基因进行克隆、测序并利用生物信息学技术分析PPV-NVP2蛋白基因的同源性、遗传进化、密码子偏爱性、糖基化位点、磷酸化位点、B细胞抗原表位及其二、三级结构。结果表明:成功扩增出包含VP2基因完整目的片段(1901bp),构建了VP2基因的克隆重组质粒pMD18-T-VP2,测序获取VP2基因序列(1740bp)并将该序列登录到GenBank(HM355807)。PPVVP2基因属高度保守的基因;PPV-N株与PPV弱毒代表毒株NADL-2株亲缘性近,推测PPV-N株属于弱毒株;PPV-N株VP2基因氨基酸密码子偏爱以A结尾的密码子;PPV-N株VP2蛋白可能存在7个糖基化位点,其丝氨酸、苏氨酸和酪氨酸可能分别有9、7、8个磷酸化位点,可能存在24个B细胞抗原表位;PPV-N株VP2蛋白二级结构预测,α-螺旋占11.74%,β-折叠占22.97%,无规则卷曲占65.28%,而三维结构预测VP2蛋白主要以无规则卷曲为主,存在多个螺旋和折叠区域。本研究结果为进一步阐释PPV-N株自然弱毒的分子机理提供依据,并为PPV分...
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| 关 键 词： | PPV-N株 VP2基因 克隆 测序 生物信息学分析 |
Cloning, Sequencing and Bioinformatics Analysis of VP2 Gene of Natural Attenuated Porcine Parvovirus N Strain |
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| Li Bin Liang Jiaxing Zhao Wu Su Qianlian He Ying Liang Baozhong Qin Yibin He Pingping.Cloning, Sequencing and Bioinformatics Analysis of VP2 Gene of Natural Attenuated Porcine Parvovirus N Strain[J].Journal of Guangxi Agricultural and Biological Science,2010(5). |
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| Authors: | Li Bin Liang Jiaxing Zhao Wu Su Qianlian He Ying Liang Baozhong Qin Yibin He Pingping |
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| Institution: | Li Bin Liang Jiaxing Zhao Wu Su Qianlian He Ying Liang Baozhong Qin Yibin He Pingping Guangxi Veterinary Research Institute; Nanning; 530001; |
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| Abstract: | In this study, we report the cloning and sequencing of VP2 gene of natural attenuated porcine parvovirus N (PPV-N) strain, and analyze the homology, heredity evolution, codon preference, glycosylation sites, phosphorylation sites, B cell epitopes, secondary structure and three-dimensional structure of VP2 protein by using bioinformatics software. The results showed that we have amplified a 1 901 base pairs (bp) fragment containing ORF of VP2 gene and constructed the recombinant plasmid pMD18-T-VP2 successfu... |
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| Keywords: | PPV-N strain VP2 gene Cloning Sequencing Bioinformatics analysis |
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