| 16份石榴RAPD扩增产物的两种电泳方法检测及其序列特征 |
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| 引用本文： | 张彦苹,曹尚银,初建青,薛华柏,王文艳,房经贵.16份石榴RAPD扩增产物的两种电泳方法检测及其序列特征[J].广西农业生物科学,2010(5). |
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| 作者姓名： | 张彦苹 曹尚银 初建青 薛华柏 王文艳 房经贵 |
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| 作者单位： | 南京农业大学园艺学院 中国农业科学院郑州果树研究所 |
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| 基金项目： | 国家自然科学基金项目(30671455)资助 |
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| 摘 要： | 本实验以16个石榴品种为实验材料,筛选出10个重复性及多态性均较好的引物进行RAPD分析。分别采用琼脂糖凝胶以及聚丙烯酰胺凝胶(PAGE)电泳检测方法对PCR扩增结果进行检测并对其结果进行比较,结果显示,两种电泳方式均能得到较为清晰的扩增条带,且两种电泳方式获得的条带总数及多态性条带数均有所不同,琼脂糖凝胶电泳方法共检测出76条带,其中有43条为多态性谱带,多态性比率为56.4%;而在PAGE电泳方法共检测出123条谱带,多态性谱带数为87条,多态性比率为70.95%。PAGE电泳方法检测出的条带数约为琼脂糖凝胶电泳方法检测出条带数的1.5倍。基于两种电泳方法所得RAPD标记的多态性位点,利用NYSYS软件计算遗传相似系数,并构建遗传关系聚类图,分析结果显示,石榴遗传多样性丰富,两种电泳方法所得聚类结果大致相同,可以利用RAPD分子标记及两种电泳检测方法对不同数量的石榴进行分子水平的品种鉴定和遗传多样性的分析。同时通过对来自几个引物随机挑选的17个片段进行克隆,测序结果显示17个片段都是对应引物的RAPD扩增产物,其中有3条是编码蛋白的基因片段,表明了RAPD不仅扩增基因组上的非编码蛋白序列,同时也可以扩增编...
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| 关 键 词： | 石榴 RAPD PAGE 序列特征 |
The Detection of 16 Pomegranate RAPD Amplified Products by Two Electrophoresis Methods and Their Sequence Characterists |
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| Zhang Yanping,Cao shangyin,Chu jianqing,Xue huabo,Wang wenyan,Fang jinggui.The Detection of 16 Pomegranate RAPD Amplified Products by Two Electrophoresis Methods and Their Sequence Characterists[J].Journal of Guangxi Agricultural and Biological Science,2010(5). |
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| Authors: | Zhang Yanping Cao shangyin Chu jianqing Xue huabo Wang wenyan Fang jinggui |
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| Institution: | 1 College of Horticulture; Nanjing Agricultural University; Nanjing; 210095; 2 Institute of Pomology in Zhengzhou of Chinese Academy of Agricultural Sciences; Zhengzhou; 450009; |
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| Abstract: | In this experiment, we chose 16 pomegranate cultivars as the experimental materials, and selected 10 RAPD primers with good reproducibility and polymorphism for analysis. Then we detect the result of PCR amplification by employing two methods of agarose gel and polyacrylamide gel electrophoresis (PAGE) gel electrophoresis and compared the results. The results showed that both this electrophoretic methods could obtain much clearer amplified bands. And the number of totall bands and the polymorphic bands are ... |
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| Keywords: | Pomegranate （Punica granatum L ） RAPD PAGE Sequence characterists |
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