| 草鱼胞浆谷胱甘肽过氧化物酶cDNA全长的克隆与分析 |
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| 引用本文： | 郑清梅,温小波,韩春艳,李浩波.草鱼胞浆谷胱甘肽过氧化物酶cDNA全长的克隆与分析[J].广西农业生物科学,2010(5). |
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| 作者姓名： | 郑清梅 温小波 韩春艳 李浩波 |
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| 作者单位： | 华南农业大学动物科学学院 嘉应学院生命科学学院 |
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| 摘 要： | 谷胱甘肽过氧化物酶(glutathione peroxidases,GPXs,EC1.11.19)是生物体内抗氧化防御系统的重要组成部分。本文从草鱼(Ctenopharyngodon idellus)克隆到胞浆谷胱甘肽过氧化物酶基因(GPX1)cDNA全长序列。该序列全长890bp(GenBank accession No.EU828796),包括完全开放阅读框(ORF)576bp、5'非编码区(5'-UTR)17bp和3'-UTR297bp。其ORF编码191个氨基酸残基,包含一个由"UGA"(通常为终止密码子)编码的硒代半胱氨酸(selenocysteine,Sec40)残基,并与另2个残基(Glu75和Trp153)构成酶活性中心。同时,草鱼GPX1cDNA的3'-UTR中具有保守的硒代半胱氨酸插入序列(selenocysteine insertion sequence,SECIS)元件。氨基酸序列相似性比较显示,草鱼GPX1cDNA的推测氨基酸序列(GenBank accession No.ACF39780)与斑马鱼GPX1(GenBank accession No.NP_001007282)的相似性...
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| 关 键 词： | 草鱼 谷胱甘肽过氧化物酶 基因克隆 组织表达 |
Full-length cDNA Cloning and Analysis of Glutathione Peroxidase from Grass Carp (Ctenopharyngodon idellus) |
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| Zheng Qingmei,;,Wen Xiaobo,Han Chunyan,Li Haobo.Full-length cDNA Cloning and Analysis of Glutathione Peroxidase from Grass Carp (Ctenopharyngodon idellus)[J].Journal of Guangxi Agricultural and Biological Science,2010(5). |
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| Authors: | Zheng Qingmei ; Wen Xiaobo Han Chunyan Li Haobo |
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| Institution: | 1 College ofAnimal Science; South China Agricultural University; Guangzhou; 510642; 2 College ofLife Science; Jiaying University; Meizhou; 514015; |
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| Abstract: | Glutathione peroxidases (GPXs, EC 1.11.19) are key enzymes in the antioxidant defence systems of liv-ing organisms. We cloned the full-length cDNAs gene of GPX1 from grass carp (Ctenopharyngodon idellus). The gene (GenBank accession No. EU828796) was 890 base-pairs (bp), including a complete protein coding region (ORF) of 576 bp, a 5 -untranslated region (5 -UTR) of 17 bp and 3 -UTR of 296 bp. The ORF encodes 191 amino acid (aa) residues containing a putative selenocysteine residue (Sec40) which is encoded ... |
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| Keywords: | Ctenopharyngodon idellus Glutathione peroxidase Gene cloning Expression |
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