| 利用微卫星标记分析马氏珠母贝4个养殖群体遗传结构 |
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| 引用本文： | 赵晓霞,邓岳文,杜晓东,王庆恒,黄荣莲,卢婧.利用微卫星标记分析马氏珠母贝4个养殖群体遗传结构[J].广西农业生物科学,2010(5). |
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| 作者姓名： | 赵晓霞 邓岳文 杜晓东 王庆恒 黄荣莲 卢婧 |
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| 作者单位： | 广东海洋大学水产学院 |
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| 基金项目： | 国家科技支撑计划(2007BAD29B01);; 农业部公益性行业科研专项经费(nyhyzx07-047);; 广东省海洋与渔业局重大科技兴海(渔)项目(A200708C01;200908A02)共同资助 |
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| 摘 要： | 2007年4月,从马氏珠母贝基础群体选取2、4、32和158个亲本分别繁殖4个子代群体,分别命名为P1、P2、P3和P4。2009年7月,从这4个子代群体随机取样30个个体,利用7对微卫星引物分析其遗传结构。结果表明,7对微卫星引物共检测到22个等位基因,每个座位的等位基因数目为2~4个,平均等位基因数为3个,平均有效等位基因数为2.3193。P1、P2、P3和P4群体平均观测杂合度分别为0.4737、0.5489、0.6767和0.7143;P1、P2、P3和P4群体平均期望杂合度分别为0.4737、0.5489、0.6767和0.7143;P1、P2、P3和P4群体的多态信息含量分别为0.4472、0.4224、0.4726和0.4930。本结果表明4个养殖群体均具有较高的遗传多样性,而且有效亲本数目对子代遗传结构有较大的影响,这为马氏珠母贝的遗传育种提供依据。
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| 关 键 词： | 马氏珠母贝 有效亲本数量 子代群体 遗传结构 |
Genetic Structure of Four Progeny Stocks of Pinctada martensii by Microsatellite Markers Analysis |
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| Zhao Xiaoxia Deng Yuewen Du Xiaodong Wang Qingheng Huang Ronglian Lu Jing.Genetic Structure of Four Progeny Stocks of Pinctada martensii by Microsatellite Markers Analysis[J].Journal of Guangxi Agricultural and Biological Science,2010(5). |
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| Authors: | Zhao Xiaoxia Deng Yuewen Du Xiaodong Wang Qingheng Huang Ronglian Lu Jing |
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| Institution: | Zhao Xiaoxia Deng Yuewen Du Xiaodong Wang Qingheng Huang Ronglian Lu Jing Fisherise College; Guangdong Ocean University; Zhanjiang; 524001; |
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| Abstract: | In April of 2007, mature individuals were sampled from the base population and used to produce four progeny stocks. The four stocks were designed as P1, P2, P3 and P4, with 2, 4, 32 and 158 individuals as breeders, respectively. In July of 2009, 30 individuals were sampled randomly from the four stocks and subjected to the experiment where the genetic variation of the stocks was evaluated by 7 microsatellite primers. The result was found that a total of 22 alleles were detected across the 7 assayed and the ... |
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| Keywords: | Pinctada martensii Effective numbers of breeders Progeny stocks Genetic variation |
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