| 一种新的耐热性植酸酶基因在烟草中的表达 |
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| 引用本文： | 高晓蓉,王国坤,王严,夏秀英,安利佳.一种新的耐热性植酸酶基因在烟草中的表达[J].安徽农业科学,2007,35(34):11019-11021. |
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| 作者姓名： | 高晓蓉 王国坤 王严 夏秀英 安利佳 |
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| 作者单位： | 大连理工大学生物科学与工程系,辽宁大连,116024;大连理工大学生物科学与工程系,辽宁大连,116024;大连理工大学生物科学与工程系,辽宁大连,116024;大连理工大学生物科学与工程系,辽宁大连,116024;大连理工大学生物科学与工程系,辽宁大连,116024 |
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| 摘 要： | 目的]探究一种新的耐热性植酸酶基因在烟草中的表达结果。方法]克隆了泡盛曲霉植酸酶基因编码区全序列,使其在烟草中高效表达,对重组植酸酶与天然植酸酶性质进行初步的分析和比较。结果]对烟草叶片的植酸酶活性检测结果表明,重组植酸酶基因在烟草中得到较高水平表达,转基因植株的最高植酸酶活力为对照烟草的13倍;对烟草重组植酸酶蛋白的酶学性质进行了初步分析,结果表明重组植酸酶拥有与天然植酸酶同样的最适pH值和最适温度,耐热性略微下降,但在80℃处理15 min后仍有33%的残余酶活力。结论]该研究为该植酸酶基因转化饲料作物的开发提供理论依据,也为泡盛曲霉植酸酶的工业化生产开辟了新的途径。
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| 关 键 词： | 泡盛曲霉 植酸酶 耐热性 转基因烟草 |
| 文章编号： | 0517-6611(2007)34-11019-03 |
| 修稿时间： | 2007年7月9日 |
Expression of a New Heat-resistant Phytase Gene in Tobacco |
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| GAO Xiao-rong.Expression of a New Heat-resistant Phytase Gene in Tobacco[J].Journal of Anhui Agricultural Sciences,2007,35(34):11019-11021. |
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| Authors: | GAO Xiao-rong |
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| Abstract: | Objective] The aim was to research the expression results of a new heat-resistant phytase gene in tobacco.Method] The primary analysis and comparison were conducted on the properties of recombinant phytase and natural phytase by cloning phytase gene coding sequence of Aspergillus awamori and making it expressed highly efficiently in tobacco.Result] The results of phytase activity detection of tobacco leaves showed that the recombinant phytase gene obtained expression with higher level in tobacco and the highest phytase activity in transgenic plant was 13 fold of that in the control tobacco.The results of primary analysis on the enzyme properties of recombinant phytase in tobacco showed that the recombinant phytase possessed the same optimum pH and optimum temperature with natural phytase.Though its heat-resistance decreased a little,it also had 33% of the residual enzyme activity after treating the recombinant phytase at 80 ℃ for 15 min.Conclusion] The research not only provided theoretical basis for the development of forage crops transformed by the phytase gene,but also opened up a new way for industrial production of phytase in Aspergillus awamori. |
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| Keywords: | Aspergillus awamori Phytase Heat-tolerance Transgenic tobacco |
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