| 液相色谱-氢化物发生原子荧光光谱测定鸡肉中 4种形态砷化合物 |
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| 引用本文： | 陈玎玎,葛高飞.液相色谱-氢化物发生原子荧光光谱测定鸡肉中 4种形态砷化合物[J].安徽农业大学学报,2017,44(3):388-391. |
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| 作者姓名： | 陈玎玎 葛高飞 |
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| 作者单位： | 安徽农业大学生物技术中心,合肥,230036;安徽农业大学生物技术中心,合肥,230036 |
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| 基金项目： | 2014校学科骨干培育项目(2014XKPY-65)资助。 |
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| 摘 要： | 建立了鸡肉中三价砷(As(Ⅲ))、一甲基砷(MMA)、二甲基砷(DMA)和五价砷(As(Ⅴ))的4种砷形态的高效液相色谱-氢化物发生原子荧光光谱(HPLC-HG-AFS)分析方法。样品采用0.15 mol·L~(-1)稀硝酸+超声波进行提取,以5 mmol·L~(-1) Na_2HPO_4和45 mmol·L~(-1) KH_2PO_4缓冲液(pH5.92)为流动相,经PRP-X100阴离子交换色谱柱分离后,原子荧光光谱测定。结果表明,4种形态的砷在8 min内实现良好分离,标准曲线的线性关系良好(r≥0.999),方法检出限为1.0~3.0mg·L~(-1),6次平行测定的相对标准偏差(RSD)为2.0%~6.2%(100mg·L~(-1))。鸡肉样品的加标回收率为82.1%~101%。
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| 关 键 词： | 砷形态 液相色谱 氢化物发生原子荧光光谱 鸡肉 |
| 收稿时间： | 2016/9/18 0:00:00 |
Simultaneous determination of four arsenic species in chicken using liquid chromatography-hydride generation atomic fluorescence spectrometry |
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| CHEN Dingding and GE Gaofei.Simultaneous determination of four arsenic species in chicken using liquid chromatography-hydride generation atomic fluorescence spectrometry[J].Journal of Anhui Agricultural University,2017,44(3):388-391. |
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| Authors: | CHEN Dingding and GE Gaofei |
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| Institution: | Biotechonlgy Center, Anhui Agricultural University, Hefei 230036 and Biotechonlgy Center, Anhui Agricultural University, Hefei 230036 |
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| Abstract: | An analytical method was developed for the simultaneous determination of four arsenic species including arsenite (As (Ⅲ)),monomethylarsonic acid (MMA),dimethylarsinic acid (DMA),and arsenate (As(V)) in chickens by liquid chromatography-hydride generation-atomic fluorescence spectrometry (HPLC-HG-AFS).The extraction of arsenic species from chicken samples was accomplished in 0.15 mol·L-1 nitric acid with ultrasonic wave.The different arsenic species were separated on a PRP-X 100 anion exchange column and analyzed by HG-AFS.The mobile phase was 5 mmol·L-1 Na2HPO4 and 45 mmol·L-1 KH2PO4 buffer (pH5.92).The separation of four arsenic species were achieved in 8 min.A good linearity was obtained for all four arsenic species,with linear correlation coefficients being greater than 0.999.The detection limit (LODs) of the four species were between 1.0 and 3.0 μg·L-1.The relative standard deviations (RSD) in six replicated measures (100 μg·L-1) varied from 2.0％ to 6.2％.The recovery rate for spiked chicken samples ranged from 82.1％ to 101％.The method is simple and sensitive with a low operation cost and environmental friendly. |
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| Keywords: | arsenic species liquid chromatography hydride generation atomic fluorescence spectrometry chicken |
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