| 云南省昆明地区种鸡J亚群禽白血病血清学调查 |
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| 引用本文： | 白马伟,赵谦,和贵斌,张正学.云南省昆明地区种鸡J亚群禽白血病血清学调查[J].安徽农业科学,2016(2). |
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| 作者姓名： | 白马伟 赵谦 和贵斌 张正学 |
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| 作者单位： | 1. 云南省蒙自市动物卫生监督所,云南蒙自661199;云南农业大学动物科学技术学院,云南昆明650201;2. 云南农业大学动物科学技术学院,云南昆明,650201 |
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| 摘 要： | 目的]了解云南省昆明地区种鸡场J亚群禽白血病的感染状况。方法]从云南省昆明市疑似感染禽白血病的某种鸡场采集644份泄殖腔(公鸡)拭子和460份蛋清(母鸡)样品,采用ELISA法对种鸡J亚群禽白血病进行抗原检测。结果]在检测的460份蛋清样品中,172份抗原呈阳性,阳性率为37.39%;在检测的644份泄殖腔拭子中,520份抗原呈阳性,阳性率为80.75%。A养殖场共检测644份样品,总阳性率为63.66%,其中蛋清样品的阳性率为39.13%,泄殖腔样品的阳性率为82.07%。B养殖场共检测460份样品,总阳性率为61.30%,其中蛋清样品的阳性率为34.78%,泄殖腔样品的阳性率为78.99%。此次检测结果表明云南省昆明地区种鸡群已严重感染J亚群禽白血病。结论]该研究可为种鸡J亚群禽白血病的防治提供理论依据。
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| 关 键 词： | 禽白血病 J亚群 p27蛋白 ELISA |
Serological Survey on J Subgroup of Avian Leukemia of Breeding Chicken in Kunming Area of Yunnan Province |
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| BAI Ma-wei,ZHAO Qian,HE Gui-bin,ZHANG Zheng-xue.Serological Survey on J Subgroup of Avian Leukemia of Breeding Chicken in Kunming Area of Yunnan Province[J].Journal of Anhui Agricultural Sciences,2016(2). |
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| Authors: | BAI Ma-wei ZHAO Qian HE Gui-bin ZHANG Zheng-xue |
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| Abstract: | Objective] To understand the infection situation of J subgroup of avian leukemia (ALV) in breeding chicken farm in Kunming City of Yunnan Province.Method] 644 cloaca swabs of cock and 460 albumen samples of hens were collected from a breeding chicken farm infected with suspected avian leukemia virus in Kunming City of Yunnan Province.The antigen of J subgroup of avian leukemia in breeding chicken was detected by using ELISA.Result] Among 460 detected albumen samples,172 antigen samples were positive,the positive rate was 37.39％.Among 644 cloaca swabs,520 antigen samples were positive,the positive rate was 80.75％.644 samples were detected in farm A,and the total positive rate was 63.66％.Among the detected samples,the positive rate of albumen samples was 39.13％,and the positive rate of cloaca swabs was 82.07％.460 samples were detected in farm B,the total positive rate was 61.30％.Among them,the positive rate of albumen samples was 34.78％,and the positive rate of cloaca swabs was 78.99％.The detection results showed that the breeding chicken in Kunming City of Yunnan Province had been severely infected with J subgroup of ALV.Conclusion] The research can provide theoretical basis for the prevention and control of J subgroup of ALV of breeding chicken. |
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| Keywords: | Avian leukemia J subgroup p27 protein ELISA |
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