| 高产淀粉酶酵母菌的筛选和鉴定 |
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| 引用本文： | 杨冬雪,孙大庆,李洪飞,李芬.高产淀粉酶酵母菌的筛选和鉴定[J].安徽农学通报,2016(8). |
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| 作者姓名： | 杨冬雪 孙大庆 李洪飞 李芬 |
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| 作者单位： | 1. 黑龙江八一农垦大学国家杂粮工程技术研究中心，黑龙江大庆 163319; 黑龙江八一农垦大学食品学院，黑龙江大庆 163319;2. 黑龙江八一农垦大学国家杂粮工程技术研究中心,黑龙江大庆,163319;3. 黑龙江八一农垦大学食品学院,黑龙江大庆,163319 |
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| 基金项目： | 大庆市指导性科技计划项目(szdfy-2015-52)；黑龙江八一农垦大学大学生创新创业训练计划项目(xc2014041)。 |
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| 摘 要： | 为了开发杂粮酵素发酵剂菌种,以淀粉酶活力为评价指标,从杂粮发酵酸面团中分离、筛选具有高产淀粉酶活力的酵母菌,并且利用分子生物学方法鉴定其菌种分类。研究结果显示,从杂粮酸面团中共分离出36株酵母菌,其中NCCC7和NCCC31菌株淀粉酶活力最高,分别为453.13U/m L和463.79U/m L,经26S r DNA鉴定,NCCC7和NCCC31均为酿酒酵母菌种。
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| 关 键 词： | 杂粮酵素 酿酒酵母 淀粉酶 |
Screening and Identification of High Amylase Producing Yeast |
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| Abstract: | In order to develop coarse cereals enzymes starter,amylase activity as the evaluation index,high amylase producing yeast were isolated and screened from coarse cereals sourdough,and classified by molecular biology tech?niques. Research results showed that 36 strains of yeast were isolated from coarse cereals sourdough,the amylase ac?tivity of NCCC7 and NCCC31 strains was the highest,453.13U/mL and 463.79U/mL respectively,and both NCCC7 and NCCC31 strains were identified as Saccharomyces cerevisiae by 26S rDNA gene. |
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| Keywords: | Coarse cereals enzymes Saccharomyces cerevisiae Amylase |
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