| 拟南芥AtHOS10基因的克隆及其表达载体的构建 |
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| 引用本文： | 王明,李翠萍,陈吉裕,苏承刚,张兴国.拟南芥AtHOS10基因的克隆及其表达载体的构建[J].南方农业,2011,5(2):30-32. |
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| 作者姓名： | 王明 李翠萍 陈吉裕 苏承刚 张兴国 |
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| 作者单位： | 南方山地园艺学教育部重点实验室,西南大学园艺园林学院,重庆,400715 |
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| 基金项目： | 重庆市科委重大攻关，农业部转基因重大专项 |
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| 摘 要： | AtHOS10基因对植物冷调节起到重要作用,可以通过控制ABA合成来改变植物的脱水胁迫耐性。本文利用PCR方法从Columbia生态型的拟南芥基因组中扩增出冷调节基因AtHOS10,并插入克隆载体。序列分析表明,克隆片段长3453bp,与目前发表的序列完全一致。将pUC-AtHOS10和pBI-HEM进行酶切重组构建表达载体pBI-AtHOS10,然后将表达载体转化导入农杆菌中,为侵染烟草作准备。
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| 关 键 词： | 拟南芥 AtHOS10基因 表达载体构建 |
Cloning and Plant Expression Vector Construction of the AtHOS10 Gene from Arabidopsis thaliana |
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| WANG Ming,LI CuiPing,CHEN JiYu,SU ChengGang,ZHANG XingGuo.Cloning and Plant Expression Vector Construction of the AtHOS10 Gene from Arabidopsis thaliana[J].South China Agriculture,2011,5(2):30-32. |
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| Authors: | WANG Ming LI CuiPing CHEN JiYu SU ChengGang ZHANG XingGuo |
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| Institution: | WANG Ming,LI CuiPing,CHEN JiYu,SU ChengGang,ZHANG XingGuo (Kay Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education,Southwest University,Chongqing 400715,China) |
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| Abstract: | AtHOS10 gene is essential for cold acclimation and may affect dehydration stress tolerance in plants by controlling stress-induced ABA biosynthesis.A cold-inducible gene AtHOS10 was amplified with PCR from the genome DNA of Arabidopsis thaliana Columbia,then constructed cloning vector,which had a length of 3453 bp and the same sequence as published previously.Expression vector pBI-AtHOS10 was constructed with pUC-AtHOS10 and pBI-HEM by digestion with restriction enzymes,then introduced into Agro-bacterium f... |
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| Keywords: | Arabidopsis AtHOS10 gene Expression vector construction |
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