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| 中国主产棉区黄萎病菌的RAPD分析 | |
| 引用本文： | 刘学堂,郭金城,张元恩,宋晓轩,孙君灵.中国主产棉区黄萎病菌的RAPD分析[J].华北农学报,1999,14(1):107-114. |
| 作者姓名： | 刘学堂 郭金城 张元恩 宋晓轩 孙君灵 |
| 作者单位： | 1. 中国农业科学院棉花研究所,安阳,455112 2. 中国农业大学植物科技学院植病系,北京 3. 农业部棉花遗传改良重点实验室,安阳 |
| 摘 要： | 在提取我国１２个省主产棉区２６个菌系ｇＤＮＡ的基础上，采用ＯＰＹ和ＯＰＢ两组随机引物，对这些菌系的基因组进行了ＰＣＲ扩增和在分析，４０个引物中，１２个引物扩增扩增出满意的适合于分析的多态性谱带。聚类分析将２６个菌纱分为Ａ、Ｂ两个群，Ｖａ菌系属于单独的Ａ群，Ｂ群则包括了所有的大丽轮枝菌系。Ｂ群又分为Ｂａ（Ｃｓ、Ｙｃ）及Ｂｂ两个亚群。Ｂｂ亚群下面分３个组，Ｂｂ１（ＡＶ２、ＡＶ３及Ｌｙ）、Ｂｂ２（Ｓａ、 |
| 关 键 词： | 棉花黄萎病菌 gDNA提取 RAPD分析 致病力测定 相关性 |
RAPD Analysis of Cotton Verticillium Wilt Pathogens |
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| Liu Xuetang,Guo Jincheng,Zhang Yuanen,Song Xiaoxuan,Sun Junling.RAPD Analysis of Cotton Verticillium Wilt Pathogens[J].Acta Agriculturae Boreali-Sinica,1999,14(1):107-114. | |
| Authors: | Liu Xuetang Guo Jincheng Zhang Yuanen Song Xiaoxuan Sun Junling |
| Abstract: | DNA extracted by CTAB method has longer fragment, higher purity and better stability. So it is suitable for the DNA extraction of cotton Verticillium wilt pathogen. The RAPD analysis has been performed with 26 cotton Verticillium strains in 40 primers (OPY, OPB), 12 primers amplified polymorphic DNA patterns. By the cluster analysis, 26 strains are divided into 2 clusters (A,B). V. alboatrum belong to cluster A, cluster B comprises the other Verticillium strains (25 strains); cluster B can be divided into 2 subclusters including Ba and Bb, subcluster Bb followed by 3 groups: Bb1, Bb2, Bb3. The group Bb3 is the largest group, including defoliating strains (T 9, V D-8 , V 44 ) and majority of Verticillium dahliae strains. The result of RAPD analysis of 4 Anyang strains showed the correlation of pathogenicity of cotton Verticillium wilt pathogen with RAPD analysis. RAPD analysis of different single spores also proved that the genomic variation may be occur among different single spores from one species. |
| Keywords: | Cotton Verticillium wilt pathogen DNA extraction RAPD analysis |
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