| 一株鸡源嗜铅乳酸菌的筛选鉴定 |
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| 引用本文： | 满兆红,都启晶,姜彦君,高雁斐,范颖华,李凤梅,赵宏坤.一株鸡源嗜铅乳酸菌的筛选鉴定[J].山东农业科学,2014(2):72-76,81. |
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| 作者姓名： | 满兆红 都启晶 姜彦君 高雁斐 范颖华 李凤梅 赵宏坤 |
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| 作者单位： | 青岛农业大学食品科学与工程学院/山东省肉类食品质量控制工程技术研究中心;山东省东营经济技术开发区安监局; |
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| 基金项目： | 国家“十二五”科技支撑计划项目“蔬菜、禽肉等生产加工与流通安全综合控制技术的集成示范”(2012BAK17B 05)、“生鲜调理肉品加工技术研发与产业化示范”(2012BAD28B03)”、“牛羊健康养殖模式构建与示范”(2012BAD39B05);山东省自主创新专项“肉鸡产品安全生产信息化和质量追溯系统集成、研究与示范”项目(2013CXC90202) |
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| 摘 要： | 试验目的是从肉鸡的肠道内容物及排泄物中筛选出具有铅抗性的细菌,用于重金属铅的去除。采用涂布平板技术从采集的样品中共分离筛选到8株具有较好铅抗性和吸附性的菌株,其中JT1菌株对铅的抗性(800 mg/L)和去除能力(66.95%)最高。通过培养特征、形态观察、生理生化鉴定、16S rDNA序列分析等方法证明,所得JT1菌株为屎肠球菌(Enterococcus faecium)。该菌株可以在10~45℃、pH 4.5~9.6、6.5%NaCl溶液、0.8%胆盐、0.1%胰蛋白酶浓度条件下生长良好。
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| 关 键 词： | 鸡 重金属 铅 乳酸菌 |
Isolation and Identification of Lead-Resistant Lactic Acid Bacteria from Chicken |
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| Man Zhaohong,Du Qijing,Jiang Yanjun,Gao Yanfei,Fan Yinghua,Li Fengmei,Zhao Hongkun.Isolation and Identification of Lead-Resistant Lactic Acid Bacteria from Chicken[J].Shandong Agricultural Sciences,2014(2):72-76,81. |
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| Authors: | Man Zhaohong Du Qijing Jiang Yanjun Gao Yanfei Fan Yinghua Li Fengmei Zhao Hongkun |
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| Institution: | 1. College of Food Science and Engineering, Qingdao Agricultural University/Shandong Engineering Research Center for Meat Food Quality Safety, Qingdao , 266109, China; 2. Safety Supervision Bureau of Dongying Economic - technological Development Area, Dongying 257091, China) |
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| Abstract: | The purpose of this study was to isolate and identify lead -resistant lactic acid bacteria from chicken intestinal content and excreta for removal of lead .Eight strains were isolated from the collected sam-ples by spread plate technique using MRS agar media supplemented with lead .The selected JT 1 strain showed the highest lead resistant （800 mg/L） and removal efficiency （66.95%）.The results of morphology observa-tion, differential cultivation, physiological and biochemical identification and 16S ribosomal deoxyribonucleic acid sequencing data showed it could be identified as Enterococcus faecium, and it could grow well under the conditions of 10~45℃, pH value as 4.5~9.6, 6.5%NaCl, 0.8%bile salts and 0.1%trypsase. |
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| Keywords: | Chicken Heavy metal Lead Lactic acid bacteria |
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