| 高DBT降解力脱硫菌株的脱硫条件优化 |
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| 引用本文： | 孙乐群,葛菁萍,王莉丽,平文祥.高DBT降解力脱硫菌株的脱硫条件优化[J].中国农学通报,2013,29(30):173-180. |
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| 作者姓名： | 孙乐群 葛菁萍 王莉丽 平文祥 |
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| 作者单位： | 1. 黑龙江大学生命科学学院2. |
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| 摘 要： | 本课题是从大庆油田土壤中分离得到一株专一性脱硫菌株HDBS-1，经鉴定，该菌种属为坂崎肠杆菌（Enterobacter sakazakii），通过诱变筛选得到了一株脱硫能力最高的菌株HDBS-4。该微生物可以按照特异性脱硫途径将DBT转化为2-HBP（2-羟基联苯）。为提高脱硫力，本试验对HDBS-4的脱硫条件及培养基进行了优化。最终确定20mL/250mL为装液量，按6%接种量， 转速为300r/min，于30℃连续培养5d。当在上述最优脱硫条件下培养时，发酵液中2-HBP生成量可达6.1105mg/L，较优化前提高了2倍多，较原始菌株提高了12倍多。
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| 关 键 词： | 乳蛋白 乳蛋白 |
| 收稿时间： | 2012/11/16 0:00:00 |
| 修稿时间： | 2013/4/16 0:00:00 |
The Optimal Desulfurization Conditions of Desulfurization Strain with High Dibenzothiophene Degradation Ability |
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| Sun Lequn,Ge Jingping,Wang Lili,Ping Wenxiang.The Optimal Desulfurization Conditions of Desulfurization Strain with High Dibenzothiophene Degradation Ability[J].Chinese Agricultural Science Bulletin,2013,29(30):173-180. |
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| Authors: | Sun Lequn Ge Jingping Wang Lili Ping Wenxiang |
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| Institution: | (Key laboratory of microbiology of Heilongjiang province/College of Life Science, Heilongfiang University, Harbin 150080) |
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| Abstract: | In order to obtain which was isolated from the a higher capability of desulfurization, a high desulfurization ability strain HDBS-4 Daqing Oilfield soil had been studied. Though observing the result of conversion dibenzothiophene into 2-hydroxybiphenyl （2-HBP）, the influence of the pH value, nitrogen source, carbon source, DBT and inoculation amount on desulfurization conditions was verified. By orthogonal test, the optimal culture conditions of HDBS-4 were further obtained. The result was showed that the best desulfurization conditions were： 25 mL cultures containing in every 250 mL medium, 6% inoculated, 30℃ in temperature. In this optimum medium, the yield of 2-HBP arrived at 6.1105 mmol/L. The strain was more than 2 times increase compared with usual condition, even 12 times than that of the original strain. The desulfurization conditions were optimized successfully at last. |
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| Keywords: | Optimum |
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