| 地衣芽孢杆菌抗菌肽的纯化及抗菌特性分析 |
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| 引用本文： | 樊陈,高兆建,张桂英,鞠民友,孙会刚,杜永凯,王东星.地衣芽孢杆菌抗菌肽的纯化及抗菌特性分析[J].中国农学通报,2013,29(33):313-318. |
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| 作者姓名： | 樊陈 高兆建 张桂英 鞠民友 孙会刚 杜永凯 王东星 |
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| 作者单位： | 1. 2. 徐州工程学院 |
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| 基金项目： | 江苏省科技创新与成果转化（重大科技支撑与自主创新）专项引导资金项目;江苏省高校自然科学基金项目;徐州市科技计划项目;大学生创新创业基金项目 |
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| 摘 要： | 以研究地衣芽孢杆菌（Bacillus licheniformis）LY12所产抗菌肽特性,从发酵液中分离纯化该抗菌肽。通过超滤、离子交换层析、分子筛凝胶过滤层析、反向高效液相色谱等方法纯化该抗菌肽。结果显示：LC-ESI-MS质谱测得抗菌肽分子量为2750.785 Da,同Tricine SDS-PAGE分析结果基本一致。体外抗菌试验结果显示,抗菌肽LYF12能够抑制丝状真菌、细菌的生长。对枯草芽孢杆菌（Bacillus subtilis）、大肠杆菌（Escherichia coli）、铜绿假单胞菌（Pseudomonas aeruginosa）、金黄色葡萄球菌（Staphylococcus aureus）、藤黄微球菌（Micrococcus aureus）、蜡状芽孢杆菌（Bacillus cereus）、副溶血性弧菌（Vibrio parachaemolyticus）的最低抑菌浓度分别为9.8、19.8、20.7、7.2、9.6、10.2、15.7μg/mL。从地衣芽孢杆菌LY12分离的抗菌肽显示出对革兰氏阴性细菌、革兰氏阳性细菌及真菌具有较好的抗菌效果,故在食品防腐中具有潜在的应用价值。
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| 关 键 词： | 抗菌肽 地衣芽孢杆菌 纯化 抑菌特性 |
| 收稿时间： | 2013/5/17 0:00:00 |
| 修稿时间： | 2013/6/10 0:00:00 |
Purification of antimicrobial peptide from Bacillus licheniformis LY12 and analysis of its antibacterial properties |
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| Fan Chen,Gao Zhaojian,Zhang Guiying,Ju Minyou,Sun Huigang,Du Yongkai,Wang Dongxing.Purification of antimicrobial peptide from Bacillus licheniformis LY12 and analysis of its antibacterial properties[J].Chinese Agricultural Science Bulletin,2013,29(33):313-318. |
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| Authors: | Fan Chen Gao Zhaojian Zhang Guiying Ju Minyou Sun Huigang Du Yongkai Wang Dongxing |
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| Institution: | 1 (1Department of Food Engineering (Bioengineering), Xuzhou Institute of Technology, Xuzhou Jiangsu 221008; 2Wanjia Animal Husbandry Science and Technology Development Co., Ltd., Xuzhou Jiangsu 221100; 3jiangsu Taihe Food Co., Ltd., Suining Jiangsu 221200) |
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| Abstract: | To explore the characterization of a novel antimicrobial peptide from from Bacillus licheniformis LY12,the antimicrobial peptide was isolated. The novel antimicrobial peptide designated LYF12,was purified,with a procedure involving ultrafiltration,ion exchange chromatography（DEAE-Sepharose FF）,gel filtration（Sephadex G-15）,and reverse phase HPLCs on C8column and C18column. LC-ESI-MS indicated the molecular mass of the purified peptide was 2750.785 Da,which was in good agreement with the result of Tricine SDS-PAGE..In vitro bioassays showed that LYF12 inhibits the growth of a variety of microbes,including filamentous fungi and bacteria. The minimum inhibitory concentrations（MIC） against Bacillus subtilis,Escherichia coli,Pseudomonas aeruginosa,Staphylococcus aureus,Micrococcus aureus,Bacillus cereus,Vibrio parachaemolyticus were 9.8,19.8,20.7,7.2,9.6,10.2,15.7 μg/mL,respectively. Because the purified antimicrobial peptide exhibited antibacterial activity against Gram-positive and Gram-negativebacteria as well as fungi,the antimicrobial peptide from Bacillus licheniformis LY12 may have potential application in food industry. |
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| Keywords: | Antibacterial properties |
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