| 基于SSR标记的赤峰地区甜荞种质的遗传多样性研究 |
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| 引用本文： | 杨学文,丁素荣,胡陶,刘迎春,张晓荣,生国利.基于SSR标记的赤峰地区甜荞种质的遗传多样性研究[J].中国农学通报,2013,29(33):129-134. |
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| 作者姓名： | 杨学文 丁素荣 胡陶 刘迎春 张晓荣 生国利 |
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| 作者单位： | 1. 赤峰市农牧科学研究院2. |
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| 基金项目： | 内蒙古自治区科技计划项目 |
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| 摘 要： | 研究内蒙古赤峰地区甜荞种质的遗传多样性,为种质资源合理利用提供依据。采用SSR分子标记对大部分来自赤峰地区的73份甜荞种质的遗传多样性进行了分析。15对SSR引物共扩增出77个条带,其中多态性条带有50条,占总数的64.9%。根据SSR扩增结果,应用NTSYSpc2.1数据分析软件计算各品种间的遗传相似系数表明,这些材料之间的遗传相似系数在0.78～0.96之间;在相似系数为0.81时,73份材料可分成4组,赤峰地区的58份材料在每一组中均有分布。赤峰地区的甜荞具有丰富的遗传多样性,具有很大的挖掘优良等位基因、进行选择育种的潜力,并发现了3份比较特异的材料。
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| 关 键 词： | 甜荞 遗传多样性 SSR标记 赤峰 |
| 收稿时间： | 2012/11/26 0:00:00 |
| 修稿时间： | 2012/12/23 0:00:00 |
SSR Marker-based Genetic Diversity of Common Buckwheat Accessions from Chifeng |
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| Yang Xuewen,Ding Surong,Hu Tao,Liu Yingchun,Zhang Xiaorong,Sheng Guoli.SSR Marker-based Genetic Diversity of Common Buckwheat Accessions from Chifeng[J].Chinese Agricultural Science Bulletin,2013,29(33):129-134. |
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| Authors: | Yang Xuewen Ding Surong Hu Tao Liu Yingchun Zhang Xiaorong Sheng Guoli |
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| Institution: | 1 (1Chifeng Academy of Agricultural and Animal Husbandary Sciences, Chifeng Inner Mongolia 024031; 2Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081; 3International Center for Bamboo and Rattan, Beijing 100102) |
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| Abstract: | Chifeng in Inner Mongolia is one of the major producing areas of common buckwheat in China. In order to study the biodiversity of common buckwheat accessions from Chifeng and make good use of the materials for breeding, we analyzed the genetic diversity and relationship among seventy-three common buckwheat accessions using fifteen pairs of SSR primers. Seventy-seven bands were amplified, fifty of which were polymorphic, which accounted for 64. 9%. Genetic coefficient of most accessions ranged from 0.78 to 0.96 by software NTSYSpc2.1 based on SSR results. The seventy-three common buckwheat accessions could be divided into four groups on coefficient threshold of 0.81. The fifty-eight accessions from Chifeng were found in all groups, indicating the high biodiversity of the accessions. |
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| Keywords: | Chifeng |
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