| 小麦三雌蕊品系UCR–14KD基因cDNA序列克隆及表达分析 |
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| 引用本文： | 蒋泽元,彭正松,杨在君,吴凯,欧阳钟鸣,巫有霞.小麦三雌蕊品系UCR–14KD基因cDNA序列克隆及表达分析[J].中国农学通报,2013,29(36):74-79. |
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| 作者姓名： | 蒋泽元 彭正松 杨在君 吴凯 欧阳钟鸣 巫有霞 |
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| 作者单位： | 1. 西华师范大学2. |
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| 摘 要： | 小麦UCR-14KD基因是呼吸链中众多重要基因之一，其表达量影响小麦呼吸速率和能量代谢，进而对小麦雌蕊和雄蕊的发育起着重要的调控作用。为进一步了解小麦UCR-14KD基因的结构特点及其在小麦三雌蕊突变体(TP)形成的分子机制中所发挥的作用，本文利用RT-PCR技术从三雌蕊突变体近等基因系CSTP幼穗中克隆了TPUCR-14KD基因完整的编码区序列，并将其转入大肠杆菌进行了诱导表达，对其cDNA及推导的氨基酸序列进行分析，结果显示：该基因编码区长381bp, 编码126个氨基酸，二级结构为α螺旋和无规卷曲，通过实时定量PCR检测，显示该基因在CSTP幼穗中大量表达，而在幼穗不同发育阶段的表达量为：幼穗长度2-5mm﹥5-7mm﹥7-10mm，并且显著高于该基因在CS幼穗对应阶段的表达量。这表明TPUCR-14KD基因可能在三粒小麦形成的过程中，尤其是在其雌蕊原基分化发育初期起到了重要作用。本研究结果对于进一步明确TPUCR-14KD基因的结构和功能，尤其是TPUCR-14KD在小麦三雌蕊形态构建中的作用奠定了基础。
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| 关 键 词： | 毛乌素沙地 毛乌素沙地 |
| 收稿时间： | 3/4/2013 12:00:00 AM |
| 修稿时间： | 2013/3/30 0:00:00 |
Sequence Cloning and Expression Analysis of UCR-14KD from the Common Wheat Line with Three Pistil |
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| Jiang Zeyuan,Peng Zhengsong,Yang Zaijun,Wu Kai,Ouyang Zhongming,Wu Youxia.Sequence Cloning and Expression Analysis of UCR-14KD from the Common Wheat Line with Three Pistil[J].Chinese Agricultural Science Bulletin,2013,29(36):74-79. |
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| Authors: | Jiang Zeyuan Peng Zhengsong Yang Zaijun Wu Kai Ouyang Zhongming Wu Youxia |
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| Institution: | (Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong Sichuan 637009) |
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| Abstract: | To study the structure and the role of wheat UCR-14KD gene in the formation process of wheat three pistils trait. The open reading frame （ORF） sequence of TPUCR-14KD gene was cloned by RT-PCR technology from young spikes from near-isogenic lines of wheat three pistils CSTP. And then the expression patterns of this gene were analyzed using real-time PCR. The open reading frame （ORF） of TPUCR-14KD was 381 bp, and encoded 126 amino acids. The secondary structure of TPUCR-14KD constituted by c~-helix and random curls. Real-time PCR analysis showed that the TPUCR-14KD gene over-expressed in young spikes of CSTP. The expression levels of： the young spike length of 2 to 5 mm〉 5 to 7 mm〉 7 to 10 mm, and the expression level was significantly higher than the corresponding stage of the young spikes in CS. The TPUCR-ldKD gene may play an important role in the process of formation of three pistil trait in common wheat line three pistil （TP）. |
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| Keywords: | Expression |
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