| 产广谱抗菌物质芽孢杆菌的筛选与鉴定 |
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| 引用本文： | 彭研,陈蕾蕾,裘纪莹,杨革.产广谱抗菌物质芽孢杆菌的筛选与鉴定[J].中国农学通报,2013,29(36):372-377. |
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| 作者姓名： | 彭研 陈蕾蕾 裘纪莹 杨革 |
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| 作者单位： | 1. 曲阜师范大学生命科学学院2. 山东省农业科学院农产品研究所3. |
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| 摘 要： | 旨在筛选出对果蔬病原菌具有广谱拮抗作用的芽孢杆菌。以9株病原真菌和3株病原细菌为指示菌，采用琼脂平板扩散法，通过初筛和复筛，筛选得到一株具有广谱抑菌作用的拮抗芽孢杆菌NCPSJ7。结合形态学、生理生化和16SrDNA序列同源性分析等手段，该菌株被鉴定为解淀粉芽孢杆菌（BacillusAmyloliquefaciens）。抑菌谱实验表明，解淀粉芽孢杆菌NCPSJ7对果蔬病原真菌和病原细菌都具有较强的抑菌能力。该菌株产抗菌物质的适宜发酵培养基、发酵条件优化，抗菌物质的分离纯化、组成、性质和抗菌稳定性等还有待于进一步研究。
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| 关 键 词： | 解淀粉芽孢杆菌 生物防治 抗菌物质 抗菌谱 16S rDNA |
| 收稿时间： | 1/6/2013 12:00:00 AM |
| 修稿时间： | 2/4/2013 12:00:00 AM |
Screening and identification of Antagonistic Bacillus producing broad-spectrum antimicrobial substances |
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| Peng Yan＇＇,Chen Leilei,Qiu Jiying,Yang Ge.Screening and identification of Antagonistic Bacillus producing broad-spectrum antimicrobial substances[J].Chinese Agricultural Science Bulletin,2013,29(36):372-377. |
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| Authors: | Peng Yan＇＇ Chen Leilei Qiu Jiying Yang Ge |
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| Institution: | 1 (＇Colledge of Life Science, Qufu normal university, Qufu Shandong 273165) (Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, J inan 250100) |
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| Abstract: | The aim was to screen Bacillus with broad antimicrobial spectrum against pathogen of fruits and vegetables. With nine pathogenic fungi and three pathogenic bacteria as indicators, Bacillus NCPSJ7 was screened using agar disk diffusion methods. Combined morphological analysis, physiological and biochemical characteristics, and 16S rDNA gene homology analysis, strain NCPSJ7 was finally identified as Bacillus Amyloliquefaciens. The antimicrobial spectrum of B. amyloliquefaciens NCPSJ7 was studied. It was shown that strain NCPSJ7 displayed both antifungal and antibacterial activity. The optimization of fermentation conditions, the composition, properties and antibacterial stability of the antibacterial substance, and separation and purification of the antibacterial substance will be studied in the further research. |
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| Keywords: | 16S rDNA analysis |
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