| 恩诺沙星诱导施氏鲟药物性肝损伤模型的建立及复方中草药保肝作用研究 |
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| 引用本文： | 栾学斌,王荻,刘红柏.恩诺沙星诱导施氏鲟药物性肝损伤模型的建立及复方中草药保肝作用研究[J].大连海洋大学学报,2017,32(1). |
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| 作者姓名： | 栾学斌 王荻 刘红柏 |
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| 作者单位： | 1. 南京农业大学 无锡渔业学院, 江苏 无锡214081;中国水产科学研究院黑龙江水产研究所, 黑龙江 哈尔滨150070;2. 中国水产科学研究院黑龙江水产研究所,黑龙江 哈尔滨,150070 |
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| 基金项目： | 国家公益性行业 (农业) 科研专项 |
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| 摘 要： | 为探究复方中草药对施氏鲟Acipenser schrenckii药物性肝损伤的保护作用,通过建立恩诺沙星肝损伤模型,在水温(15.5±1)℃下对3组自组中草药复方的保肝作用进行了筛选比较研究,试验设5组,对空白对照组及模型对照组1+龄施氏鲟以每天2%体质量投喂基础饲料,对3组复方中草药组施氏鲟投喂相应中草药药饵,试验进行至第11天时,复方中草药组及模型对照组分别口灌160 mg/kg(体质量)恩诺沙星,连续10 d,空白对照组以等量双蒸水代替,口灌期间各组仍按原方式继续投喂(基础饲料或药饵),试验结束后,测定各组血清中与肝损伤相关的活性物质含量及酶活性,并观察肝组织病理变化情况。结果表明:与空白对照组相比,模型对照组供试鲟血清中T-AOC能力显著下降(P0.05),T-SOD活力极显著下降(P0.01),ALT、AST活力和MDA含量极显著上升(P0.01),肝组织切片显示,器质性病变明显,肝损伤建模成功;3组复方中草药均可升高供试鲟血清中T-SOD活力、T-AOC能力,降低MDA含量和AST、ALT活力;供试鲟血清生化指标同其肝组织病理变化趋势相吻合。研究表明,3组复方中草药均能有效减轻恩诺沙星致施氏鲟肝细胞损伤,并对肝细胞恢复起到积极作用,尤其复方三中草药效果明显。
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| 关 键 词： | 施氏鲟 中草药 肝损伤 恩诺沙星 |
A model of drug-induced liver injury caused by enrofloxacin and protective effects of compound Chinese herbal medicines on livers in Amur sturgeon Acipenser schrenckii |
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| Authors: | LUAN Xue-bin WANG Di LIU Hong-bai |
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| Abstract: | After a trial on an inducing sturgeon liver injury model ( M group) via requirement time and dose test, Amur sturgeon Acipenser schrenckii with body weight of (100±2) g were reared in a circular cement pond and fed the basal diet in the control group and diets containing Chinese herbal medicine compound ( Chinese herbal medi-cine groups, CHM groups and drug-induced liver injury model group, M group) at daily feeding rate of 2% at wa-ter temperature of (15. 5±1. 0)℃ for 11 days. Then enrofloxacin was given to the Amur sturgeon in CHM groups and M group at a dose of 160 mg/kg body weight and equivalent double distilled water was given to the Amur stur-geon in control group by oral administration for 10 days to explore the protective effect of compound Chinese herbal medicines on drug-induced liver injury in Amur sturgeon. The levels of hepatic active materials and activities of enzymes in serum were detected and the hepatic histopathology was observed in the Amur sturgeon administrated a-bove. Results showed that there were significantly lower serum level of total antioxidant capacity ( T-AOC) ( P<0. 05) and very significantly lower total superoxide dismutase (T-SOD) activities(P<0. 01), and very significant-ly higher activities of glutamic-pyruvic transaminase ( ALT) , and glutamic oxalacetic transaminase ( AST) and ma-londialdehyde (MDA) level(P<0. 01) in the Amur sturgeon in M group than those in the control group, with obvi-ous liver histopathology under a microscope, indicating that the liver was injured obviously and the liver injury mod-el was established successfully. The Amur sturgeon in CHM groups had higher serum level of T-AOC and activities of T-SOD, and lower serum level of MDA and activities of AST and ALT, with the same characteristics of hepatic histopathology. The findings proved that Chinese herbal medicines inhibited the physical absorption of enrofloxacin, improved the ability of scavenging free radicals, and promoted hepatocyte recovery, especially compound Ⅲ. |
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| Keywords: | Acipenser schrenckii Chinese herbal medicine liver injury enrofloxacin |
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