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盐度及pH突变对大竹蛏稚贝抗氧化酶活力的影响
引用本文:高杉,周遵春,董颖,曹琛,王摆,姜北,张乾,匡少华,郝咏芳.盐度及pH突变对大竹蛏稚贝抗氧化酶活力的影响[J].大连海洋大学学报,2017,32(1).
作者姓名:高杉  周遵春  董颖  曹琛  王摆  姜北  张乾  匡少华  郝咏芳
作者单位:1. 辽宁省海洋水产科学研究院 辽宁省海洋水产分子生物学重点实验室,辽宁 大连,116023;2. 营口市水产科学研究所,辽宁营口,115000
基金项目:现代农业产业技术体系建设专项,辽宁省科技计划项目,辽宁省海洋与渔业厅项目
摘    要:为了解盐度、pH对大竹蛏Solen grandis稚贝抗氧化酶活力的影响,在不同盐度(15、20、30、35)和pH(7.0、7.5、8.5、9.0)突变下,分别在0、4、12、24、48、72、96 h测定了壳长为(1.33±0.10)cm大竹蛏稚贝超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活力的变化。结果表明:盐度20组12、24h及盐度30组24 h,大竹蛏稚贝SOD活力显著高于对照组(盐度25)(P0.05),盐度20组4、12 h和盐度30组12、24 h时,大竹蛏稚贝CAT活力显著高于对照组(P0.05),48~96 h时,盐度20、盐度30组的两种抗氧化酶活力逐渐恢复至对照组水平;盐度15、35组48~96 h时,大竹蛏稚贝的SOD和CAT活力显著高于对照组(P0.05),pH 7.5、pH 8.5组12~96 h时,大竹蛏稚贝的SOD和CAT活力显著高于对照组(pH 8.0)(P0.05)(除pH 8.5组48 h外);pH 7.0、pH 9.0组12~96 h时,其SOD活力显著高于对照组(P0.05),pH 7.0和pH 9.0组72 h和96 h时,CAT活力显著低于对照组(P0.05)。研究表明:盐度和pH突变对大竹蛏稚贝SOD和CAT活力均有影响,大竹蛏稚贝对盐度20和盐度30突变有一定适应性;盐度15、盐度35和pH 7.5、pH 8.5突变后,大竹蛏稚贝长时间处于氧化应激状态;pH 7.0和pH 9.0突变可能会造成大竹蛏稚贝细胞氧化损伤;生产实践中,大竹蛏稚贝培育的盐度和pH范围分别控制在20~30和7.5~8.5较为合适。

关 键 词:大竹蛏  盐度  pH  超氧化物歧化酶  过氧化氢酶

Effects of changes in salinity and pH on antioxidant enzyme activity in juvenile razor clam Solen grandis
Abstract:Superoxide dismutase ( SOD) and catalase ( CAT) activities of juvenile razor clam Solen grandis with shell length of(1. 33 cm±0. 10 cm) were monitored at a salinity of 15, 20, 30 and 35 and pH of 7. 0, 7. 5, 8. 5 and 9. 0 for 0, 4, 12, 24, 48, 72 and 96 h, respectively, in order to clarify the effects of salinity and pH on the antioxidant enzyme activities of juvenile razor clam. The results showed that there were significantly higher activities of SOD in the clam exposed to a salinity of 20 for 12 and 24 h and a salinity of 30 for 24 h than those in the control group (salinity 25) (P<0. 05). However, there were significantly higher CAT activities in the clam exposed to a salinity of 20 for 4 and 12 h and a salinity of 30 for 12 and 24 h than those in the control group ( salinity 25 ) ( P<0. 05), finally activities of both SOD and CAT being recovered to the control level in 48 h and 96 h. The razor clam exposed to a salinity of 15 and 35 from 48 h to 96 h had significantly higher SOD and CAT activities than those in controls (P<0. 05) did. The razor clam exposed to pH 7. 5 and pH 8. 5 from 12 h to 96 h had significantly high-er SOD and CAT activities than those in control group did(pH 8. 0, except pH 8. 5 in 48 h) (P<0. 05). There was significantly higher SOD activity in the clam exposed to pH 7 . 0 and pH 9 . 0 than those in the control group at 12-96 h ( P<0 . 05 ) , the activities of CAT being significantly lower from 72 h to 96 h ( P<0 . 05 ) . The findings in-dicated that SOD and CAT activities in razor clam were affected by changes in salinity and pH, the razor clam being adaptable to sudden change in salinity within 20 and 30 , and that the razor clam showed cellular oxidative damage induced by the pH 7 . 0 and pH 9 . 0 changes under the status of oxidative stress for a long period of exposure at a sa-linity of 15 and 35 and pH 7 . 5 , and 8 . 5 . The adaptive salinity and pH are suggested to should be kept in 20-30 and 7. 5-8. 5 for the culture of Solen grandis juveniles, respectively.
Keywords:Solen grandis  salinity  pH  superoxide dismutase ( SOD)  catalase ( CAT)
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