| 绵羊FecB基因打靶载体构建 |
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| 引用本文： | 吴伟伟,王力俭,田可川,徐新明,张廷虎,张艳花,哈尼克孜.绵羊FecB基因打靶载体构建[J].畜牧与兽医,2011,43(7). |
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| 作者姓名： | 吴伟伟 王力俭 田可川 徐新明 张廷虎 张艳花 哈尼克孜 |
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| 作者单位： | 新疆畜牧科学院,新疆乌鲁木齐,830000 |
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| 基金项目： | 国家绒毛用羊产业技术体系、自治区重大专项(200731132-7); 农业科技成果转化资金项目(2008GB2G400352) |
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| 摘 要： | 利用peGFP-C1,pRET.IL.PGK-TK和pMD19-T Simple等基本质粒,构建绵羊FecB基因打靶载体。以克隆载体pMD19-T Simple为载体骨架,插入一个正选择标记eGFP基因、一个负选择标记基因HSV-tk基因,并在eGFP基因两侧各添加一个同源长臂和同源短臂,从而构建绵羊FecB基因打靶载体。结果:经多个限制性内切核酸酶酶切鉴定和测序证实,所构建的基因打靶载体符合设计要求,表明成功构建了绵羊FecB基因打靶载体。
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| 关 键 词： | FecB基因 基因打靶 载体构建 绵羊 |
Construction of a new gene targeting vector in the sheep |
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| WU Wei-wei,WANG Li-jian,TIAN Ke-chuan,XU Xin-ming,ZHANG Ting-hu,ZHANG Yan-hua,HA Ni-kezi.Construction of a new gene targeting vector in the sheep[J].Animal Husbandry & Veterinary Medicine,2011,43(7). |
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| Authors: | WU Wei-wei WANG Li-jian TIAN Ke-chuan XU Xin-ming ZHANG Ting-hu ZHANG Yan-hua HA Ni-kezi |
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| Institution: | WU Wei-wei,WANG Li-jian,TIAN Ke-chuan,XU Xin-ming,ZHANG Ting-hu,ZHANG Yan-hua,HA Ni-kezi(Xinjiang Academy of Animal Science,Urumqi 830000,China) |
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| Abstract: | In order to construct a sheep FecB gene targeting vector,plasmids of peGFP-C1,pRET.IL.PGK-TK and pMD19-T Simple and pMD19-T Simple cloning vector as the carrier frame with a positive selection marker eGFP gene and a negative selection marker gene HSV-tk gene were used.The correct structure of the targeting vector was confirmed by restriction enzyme digestion and sequencing analysis.Our experiment indicated that a FecB gene targeting vector of sheep was successfully constructed. |
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| Keywords: | FecB gene gene targeting vector construction sheep |
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