| 长江下游铜鱼线粒体DNA（mtDNA）遗传多样性的PCR-RFLP分析 |
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| 引用本文： | 张敏莹.长江下游铜鱼线粒体DNA（mtDNA）遗传多样性的PCR-RFLP分析[J].中国农学通报,2009,25(22):352-355. |
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| 作者姓名： | 张敏莹 |
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| 作者单位： | 农业部长江下游渔业资源环境重点野外科学观测试验站,中国水产科学研究院内陆渔业生态环境和资源重点开放实验室,中国水产科学研究院淡水渔业研究中心,江苏无锡,214081 |
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| 基金项目： | 国家科技基础条件平台项目"水产种质资源标准化整理整合与共享"，水产科学研究院专项资金项目"长江下游稀缺物种资源与生态评估"，"蠡湖鱼类多样性及种质资源特性研究" |
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| 摘 要： | 对长江下游铜鱼线粒体DNA控制区（D-Loop区）和细胞色素b（Cytb）基因进行PCR扩增,扩增片段用10种限制性内切酶酶切。PCR-RFLP研究结果表明,D-Loop区和Cytb基因均未表现出个体之间的长度变异现象。10种核酸内切限制酶中,有2种对Cytb基因有酶切位点,有3种对D-Loop区有酶切位点。有酶切位点的5种限制性内切酶共检测到3种线粒体DNA单倍型。在Cytb基因检测到铜鱼个体间的变异,在D-Loop区没有检测到铜鱼个体间的变异。长江下游铜鱼线粒体DNA单倍型多样性指数（h）为0.6071,线粒体DNA遗传多样性较低。
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| 关 键 词： | 铜鱼 线粒体DNA 控制区 细胞色素b基因 遗传多样性 限制性片段长度多态 |
| 收稿时间： | 2009/6/11 0:00:00 |
| 修稿时间： | 2009/7/15 0:00:00 |
Analysis on Genetic Diversity of mtDNA of Coreius heterodon in the Lower Reaches of the Yangtze River by PCR-RFLP |
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| Zhang Minying,Duan Jinrong,Xu Dongpo,Liu Kai,Shi Weigang.Analysis on Genetic Diversity of mtDNA of Coreius heterodon in the Lower Reaches of the Yangtze River by PCR-RFLP[J].Chinese Agricultural Science Bulletin,2009,25(22):352-355. |
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| Authors: | Zhang Minying Duan Jinrong Xu Dongpo Liu Kai Shi Weigang |
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| Institution: | (Key Field Station of Observation and Research for Fishery Resources and Environment of the Lower Reaches of Yangtze River; Ministry of Agriculture, Key Laboratory of Ecological Environment and Resources of Inland Fisheries; Freshwater Fisheries Research Center, CAFS, Wuxi Jiangsu 214081) |
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| Abstract: | Mitochondrial control （D-Loop）region and cytochrome b （Cytb） gene of Bronze gudgeon （Coreius heterodon） in the lower reaches of the Yangtze River were cloned by PCR . Cloned fragments were digested by ten kinds of restriction endonuclease. Result of PCR-RFLP showed that no length diversity among different individual were detected both in D-Loop region and Cytb gene. Among ten restriction endonucleases, Cytb gene could be digested by two kinds of those and D-Loop region could be digested by three kinds of those. Three kinds of haplotypes were detected with five enzymes. Diversity among individuals of Bronze gudgeon was detected in Cytb gene, while no diversity was found among different individuals in D-Loop region. The haplotype diversity index of Bronze gudgeon in the lower reaches of the Yangtze River was 0.6071, which revealed a lower genetic diversity in mtDNA. |
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| Keywords: | Bronze gudgeon| mtDNA| D-Loop region| Cytb gene|Genetic diversity| RFLP |
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