| 不同倍性团头鲂群体遗传变异的初步分析 |
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| 引用本文： | 唐首杰,李思发.不同倍性团头鲂群体遗传变异的初步分析[J].上海水产大学学报,2007,16(2):97-102. |
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| 作者姓名： | 唐首杰 李思发 |
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| 作者单位： | 上海水产大学农业部水产种质资源与养殖生态重点开放实验室,上海200090 |
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| 基金项目： | 上海市科技兴农科技攻关项目 |
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| 摘 要： | 从鲤的微卫星DNA标记中筛选4对有效引物，进行不同倍性团头鲂遗传变异的微卫星DNA分析。结果表明：（1）4对引物中有2对能在不同倍性团头鲂五群体中探测到多态性，其等位基因数为4～6个，大小在131～307bp之间。发现两个等位基因（MFW19—175和MFW19—256）可作为异源3n特异的微卫星DNA标记。（2）不同倍性团头鲂五群体内的Shannon多样性指数为0．0562～0．2887，Nei’s基因多样性指数为0．0315～0．1969，平均遗传距离为0．0395～0．1542，不同倍性团头鲂群体存在较大的遗传变异。（3）反交3n、异源3n、正交3n和同源4n—F1群体的Shannon多样性指数和Nei’s基因多样性指数均显著地高于2n群体（P〈0．05）。（4）分子方差分析和聚类分析的结果表明：五群体分子系统树分成明显的两支，同源4n—F1、正交3n、反交3n和异源3n为一支，2n为另一支。
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| 关 键 词： | 团头鲂 倍性 微卫星 遗传变异 |
| 文章编号： | 1004-7271（2007）02-0097-06 |
| 收稿时间： | 2006-06-20 |
| 修稿时间： | 2006-06-20 |
Preliminary analysis of genetic variation of blunt snout bream ( Megalobrama amblycephala) populations with different ploidy levels |
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| TANG Shou-jie,LI Si-fa.Preliminary analysis of genetic variation of blunt snout bream ( Megalobrama amblycephala) populations with different ploidy levels[J].Journal of Shanghai Fisheries University,2007,16(2):97-102. |
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| Authors: | TANG Shou-jie LI Si-fa |
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| Institution: | Key Laboratory of Aquatic Genetic Resources and Aquacultural Ecosystem Certificated by the Ministry of Agriculture Shanghai Fisheries University,Shanghai 200090, China |
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| Abstract: | The molecular genetic variation of blunt snout bream(Megalobrama amblycephala) with different ploidy levels was investigated using 4 pairs of microsatellite primers.The results showed that:(1)In 4 primers,only 2 primers were effective,and 4-6 alleles were detected by each primer,and the size of alleles ranged from 131 bp to 307 bp.Meanwhile,two alleles(MFW19-175 and MFW19-256) were found as specific microsatellite DNA markers to distinguish allo-3n from the other four populations.(2)Shannon's diversity index,Nei's gene diversity index and the average genetic distance within each population were 0.056 2-0.288 7,0.031 5-0.196 9 and 0.039 5-0.154 2 respectively,which was a good indication of high genetic variation in five populations of blunt snout bream with different ploidy levels.(3)Both Shannon's diversity index and Nei's gene diversity index from Negative 3n hybrid,Allo-3n,Positive 3n hybrid and Auto-4n-F1 were significantly higher than those of 2n population(P<0.05).(4)Analysis of molecular variance(AMOVA) and cluster analysis indicated that there was significant divergence among five populations of blunt snout bream with different ploidy levels.The phylogenetic tree has two independent evolutionary branches.Negative 3n hybrid,Allo-3n,Positive 3n hybrid and Auto-4n-F1 shared a common group,while 2n was another independent evolutionary branch. |
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| Keywords: | blunt snout bream(Megalobrama amblycephala) ploidy microsatellite genetic variation |
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