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哈茨木霉T2-16发酵产物对豇豆种子细胞膜及细胞保护酶系统的影响
引用本文:魏林,梁志怀,曹福祥,陈玉荣.哈茨木霉T2-16发酵产物对豇豆种子细胞膜及细胞保护酶系统的影响[J].种子,2011,30(3).
作者姓名:魏林  梁志怀  曹福祥  陈玉荣
作者单位:1. 中南林业科技大学,湖南,长沙,410004;湖南省植物保护研究所,长沙,410125
2. 湖南省植物保护研究所,长沙,410125
3. 中南林业科技大学,湖南,长沙,410004
基金项目:湖南省博士后科研资助专项计划项目,农业部公益性行业(农业)科研专项经费项目
摘    要:应用哈茨木霉T2-16菌株在改良后的GPF培养基中发酵培养获得的发酵产物,对豇豆种子进行浸种处理,分析了发酵产物在种子萌发过程中主要生理生化过程的影响.结果表明,该发酵产物可增强豇豆体内超氧化歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)等保护酶活性,有效保护种子细胞膜结构的完整性,减少了氨基酸、蛋白质、可溶性糖等胞内营养物质的外渗,从而起到提高豇豆种子活力的效果.

关 键 词:哈茨木霉  发酵产物  豇豆  种子活力

Effects of the Fermented Products of Trichoderma harzianum T2-16 on the Cell Membrane and Cell Protective Enzyme System of Cowpea Seeds
WEI Lin,LIANG Zhi-huai,CAO Fu-xiang,CHEN Yu-rong.Effects of the Fermented Products of Trichoderma harzianum T2-16 on the Cell Membrane and Cell Protective Enzyme System of Cowpea Seeds[J].Seed,2011,30(3).
Authors:WEI Lin  LIANG Zhi-huai  CAO Fu-xiang  CHEN Yu-rong
Institution:WEI Lin1,2,LIANG Zhi-huai2,CAO Fu-xiang1,CHEN Yu-rong2(1.Central South University of Forestry and Technology,Changsha Hu'nan 410004,China,2.Hunan Provincial Plant Protection Institute,Changsha Hu'nan 410125,China)
Abstract:The fermented product of T.harzianum T2-16 strain was applied on cowpea by seed soaking.The effects on the seeds vigor were studied.The results showed that the specific activities of protective enzymes,superoxide dismutase(SOD),catalase(CAT) and peroxidase(POD)all increased.The fermented substance could also make the cell membrane permeability,decrease the conductivity rate.The contents of protein,dissoluble sugar and amino acids in cell exudates of cowpea seedlings were less than that of treated by water.T...
Keywords:Trichoderma harzianum  fermented product  cowpea  seeds vigor  
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