| 棉花基因组DNA快速提取方法改良 |
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| 引用本文： | 李忠旺,厚毅清,石有太,陈玉梁.棉花基因组DNA快速提取方法改良[J].甘肃农业科技,2012(4):11-13. |
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| 作者姓名： | 李忠旺 厚毅清 石有太 陈玉梁 |
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| 作者单位： | 甘肃省农业科学院生物技术研究所,甘肃兰州,730070 |
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| 基金项目： | 国家转基因生物新品种培育重大专项“西北干旱区转基因抗旱彩色棉新种质创制研究” |
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| 摘 要： | 选择2种简化的棉花基因组DNA提取方法并进行适当精简改良后与传统CTAB提取法进行实验对比的结果显示,改良SDS-CTAB法所提DNA得率较高,纯度好,但其操作步骤相对复杂。改良CTAB法操作更加简捷,但DNA得率相对较低,所得DNA溶液浑浊度较高。相对于传统的CTAB提取法,两种改良方法都能够更加快捷的得到足量且纯净的基因组DNA,完全能够满足转基因后代PCR筛选检测的要求。
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| 关 键 词： | 棉花 基因组 DNA SDS法 CTAB法 |
An Improvement Method of DNA Rapid Extraction For Cotton Genomic |
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| LI Zhong-wang,HOU Yi-qing,SHI You-tai,CHEN Yu-liang.An Improvement Method of DNA Rapid Extraction For Cotton Genomic[J].Gansu Agricultural Science and Technology,2012(4):11-13. |
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| Authors: | LI Zhong-wang HOU Yi-qing SHI You-tai CHEN Yu-liang |
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| Institution: | (Institute of Biotechnology,Gansu Academy of Agricultural Science,Lanzhou Gansu 730070,China) |
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| Abstract: | In order to explore a fast,simple,and especially reliable cotton genomic DNA extraction method,two traditional simple methods were improved and compared in experiment.The results showed that the improved SDS-CTAB method was characteristic of clean solution and DNA high yield,but there was more experiment steps in the method.The improved CTAB method has fewer steps,and was suit to large number of samples extraction of multiple batch cycle process,but DNA yield was lower and solution was not clean.The results demonstrated that two improved method,comparing to traditional CTAB methods of total DNA isolation in cotton,can get quickly sufficient pure genomic DNA,which was sufficient for PCR screening in transgenic descendant detection. |
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| Keywords: | Cotton Genomic DNA SDS method CTAB method |
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