| 菊花ClERF1基因的克隆与表达分析 |
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| 引用本文： | 任镘蓉,全英杰,岳圆圆,杨文婷,何子涵,高日.菊花ClERF1基因的克隆与表达分析[J].北方园艺,2021(1):66-72. |
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| 作者姓名： | 任镘蓉 全英杰 岳圆圆 杨文婷 何子涵 高日 |
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| 作者单位： | 延边大学农学院,吉林延吉133002;延边大学农学院,吉林延吉133002;延边大学农学院,吉林延吉133002;延边大学农学院,吉林延吉133002;延边大学农学院,吉林延吉133002;延边大学农学院,吉林延吉133002 |
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| 基金项目： | 吉林省"十三五"科学技术资助项目;延边大学博士科研启动基金资助项目;农业农村部景观设计重点实验室开放课题资助项目;国家自然科学基金地区科学基金资助项目;延边大学青年基金资助项目 |
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| 摘 要： | 以甘菊叶片为试验材料,采用RT-PCR和Tail-PCR以及生物信息学的方法,研究了甘菊ClERF1及其启动子序列特征、表达模式,以及甘菊ClERF1在低温胁迫下的响应。以期为进一步研究甘菊ClERF1功能提供参考依据。结果表明:ClERF1基因序列全长1 242 bp,最大开放阅读框(ORF)618 bp,可编码206个氨基酸。经理化性质分析,ClERF1分子量23 631.35 Da,理论等电点5.19,不稳定系数53.84,脂肪指数62.04,平均亲水性-0.786,亚细胞定位在细胞核内。系统进化树表明,ClERF1与拟南芥At3g23240亲缘关系最近,属于ERF亚家族。qRT-PCR分析表明ClERF1在叶片中表达最高,其次在茎段中。该研究克隆了ClERF1启动子序列1 534 bp,主要包括低温反应和乙烯响应元件、生长素和茉莉酸甲酯反应元件等。甘菊低温处理幼苗ClERF1表达量显著高于未低温处理的幼苗,其中5℃时ClERF1相对表达量最高。
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| 关 键 词： | 菊花 AP2/ERF 基因克隆 启动子元件 |
Cloning and Expression Analysis of ClERF1 Gene in Chrysanthemum |
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| REN Manrong,QUAN Yingjie,YUE Yuanyuan,YANG Wenting,HE Zihan,GAO Ri.Cloning and Expression Analysis of ClERF1 Gene in Chrysanthemum[J].Northern Horticulture,2021(1):66-72. |
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| Authors: | REN Manrong QUAN Yingjie YUE Yuanyuan YANG Wenting HE Zihan GAO Ri |
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| Institution: | (College of Agriculture,Yanbian University,Yanji,Jilin 133002) |
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| Abstract: | The leaves of chamomile were used as the test material,RT-PCR,Tail-PCR and bioinformatics methods were used to study the characteristics and expression patterns of the promoter sequences of ClERF1 and its response to low temperature stress.In order to provide a theoretical basis for further study on the function of chamomile ClERF1.The results showed that ClERF1 gene sequence was 1 242 bp,with a maximum open reading frame(ORF)of 618 bp,encoding 206 amino acids.Manager property analysis showed that ClERF1 molecular weight 23 631.35 Da,theoretical pI5.19,instability index 53.84,aliphatic index 62.04,grand average of hydrophilicity-0.786,and subcellular location in the nucleus.Phylogenetic tree showed that ClERF1 was closely related to arabidopsis At3 g23240 and belonged to the ERF subfamily.qRT-PCR analysis showed that ClERF1 expressed the highest expression in leaves,followed by in stem segments.ClERF1 promoter sequence of 1 534 bp was cloned,which mainly included low temperature reaction and ethylene response element,auxin and methyl jasmonate reaction element.The expression level of ClERF1 in chamomile seedlings treated at different low temperatures was significantly higher than that in the seedlings not treated at low temperatures,and the relative expression level of ClERF1 was the highest at 5℃. |
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| Keywords: | chrysanthemum AP2/ERF gene cloning promoter element |
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