| 铁皮石斛人工繁育技术 |
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| 引用本文： | 黄艳艳,仉劲,付均惠,张攀,秦宁,李国清.铁皮石斛人工繁育技术[J].北方园艺,2021(2):115-123. |
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| 作者姓名： | 黄艳艳 仉劲 付均惠 张攀 秦宁 李国清 |
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| 作者单位： | 泰安市泰山林业科学研究院,山东泰安271000;泰安市泰山林业科学研究院,山东泰安271000;泰安市泰山林业科学研究院,山东泰安271000;泰安市泰山林业科学研究院,山东泰安271000;泰安市泰山林业科学研究院,山东泰安271000;泰安市农业科学研究院,山东泰安271000 |
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| 基金项目： | 山东省农业良种工程资助项目;2018年中医药公共卫生服务补助专项资助项目 |
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| 摘 要： | 以铁皮石斛商品瓶苗和盆栽苗茎段为试材,添加不同浓度6-BA、NAA,诱导茎段腋芽、原球茎和不定根,建立再生体系,快速繁殖铁皮石斛组培苗,以期为秦岭淮河一线以北地区人工繁殖铁皮石斛提供参考依据。结果表明:外植体宜采用70%酒精浸泡20 s,0.1%升汞浸泡8 min消毒;MS+6-BA 3.0~5.0 mg·L^-1+NAA 0.1~1.0 mg·L^-1适合腋芽分化和增殖,分化率92%~100%;1/2MS+6-BA 2.0 mg·L^-1+NAA 0.5 mg·L^-1+土豆泥15%适合原球茎诱导增殖,增殖倍数7.7;1/2MS+NAA 0.3 mg·L^-1生根率为100%;以松针土移栽,成活率83%。
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| 关 键 词： | 铁皮石斛 再生体系 人工繁殖 |
Artificial Propagation Technology of Dendrobium officinale |
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| HUANG Yanyan,ZHANG Jin,FU Junhui,ZHANG Pan,QIN Ning,LI Guoqing.Artificial Propagation Technology of Dendrobium officinale[J].Northern Horticulture,2021(2):115-123. |
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| Authors: | HUANG Yanyan ZHANG Jin FU Junhui ZHANG Pan QIN Ning LI Guoqing |
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| Institution: | (Tai′an Academy of Forestry Sciences,Tai'an,Shandong 271000;Tai′an Academy of Agricultural Sciences,Tai'an,Shandong 271000) |
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| Abstract: | In order to provide technical reference for artificial breeding of Dendrobium officinale situated areas north,taking purchased tissue culture seedlings and potted seedlings as test materials,different concentrations of 6-BA and NAA were used to induce axillary buds,protocorms and adventitious roots to establish regeneration system,rapid propagation tissue culture seedlings of Dendrobium officinale.The results showed that the explants should be disfected by 70%alcohol for20 seconds and 0.1%mercuric chloride for 8 minutes.MS+6-BA 3.0-5.0 mg·L^-1+NAA 0.1-1.0 mg·L^-1 was suitable for axillary buds differentiation and proliferation.The differentiation rate was 92%-100%;1/2 MS medium with 6-BA 2.0 mg·L^-1+NAA 0.5 mg·L^-1+mashed potato15%was suitable for protocorms proliferation with a proliferation multiple of 7.7.In 1/2 MS medium with NAA 0.3 mg·L^-1,the rooting rate was 100%.Transplanting with pine needle soil,the survival rate was 83%. |
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| Keywords: | Dendrobium officinale regeneration system artificial propagation system |
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