| 桑原生质体分离技术的研究 |
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| 引用本文： | 陈爱玉,王勇.桑原生质体分离技术的研究[J].蚕业科学,1994,20(3):141-144. |
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| 作者姓名： | 陈爱玉 王勇 |
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| 作者单位： | 中国农业科学院蚕业研究所 |
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| 摘 要： | 用桑子叶、桑幼叶、悬浮培养细胞、愈伤组织四种材料，分别进行了原生质体的分离方法及有关条件的研究。结果在适宜酶溶液浓度下，桑原生质体的释放速度和产量顺序为，桑子叶＞幼叶＞悬浮培养细胞＞愈伤组织。酶液最适渗透剂浓度为０．５－０．６Ｍ的甘露醇。经预培养处理的材料，原生质体产量比对照提高１２．９倍。
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| 关 键 词： | 桑树，原生质体，分离 |
STUDIES ON MULBERRY PROTOPLAST ISOLATION TECHNIQUES |
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| Chen Ai-yu Wang Yong Ni,Guo-fu.STUDIES ON MULBERRY PROTOPLAST ISOLATION TECHNIQUES[J].Acta Sericologica Sinica,1994,20(3):141-144. |
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| Authors: | Chen Ai-yu Wang Yong Ni Guo-fu |
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| Abstract: | Mulberry cotyledons,young leaves,suspension cells and calli were used
for proto-plast isolation studies. The results showed that :1) Under appropriate enzyme
concentra-tion,the release speed and yield of mulberry protoplasts was in the order of
cotyledons>young leaves > suspension cells>calli;2) The optimum osmotic concentration
forenzyme solution was 0.5～0.6M tnannitol;3) Pretreatment to young leaves increasedprotoplast
yield by 12. 9 times. |
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| Keywords: | Mulberry Protoplast lsolation |
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