| 不同介质对海滨斯氏线虫抗紫外胁迫的影响 |
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| 引用本文： | 肖建飞,韩岚岚,赵奎军,郝子茹,朱 琳,师正浩,高丽瞳,陈 娟.不同介质对海滨斯氏线虫抗紫外胁迫的影响[J].植物保护,2021,47(3):115-121. |
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| 作者姓名： | 肖建飞 韩岚岚 赵奎军 郝子茹 朱 琳 师正浩 高丽瞳 陈 娟 |
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| 作者单位： | 东北农业大学农学院, 哈尔滨 150030 |
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| 基金项目： | 国家现代农业产业技术体系建设专项（CARS-04）；国家重点研发计划(2017YFD0201000) |
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| 摘 要： | 为了筛选合适的介质以提高昆虫病原线虫抵抗紫外胁迫的能力,本文研究了储存于木质素磺酸钙、预胶化淀粉、蛭石、黑炭和无菌蒸馏水(CK)中的海滨斯氏线虫Steinernema litorale在距18 W紫外灯30 cm处照射30、60、90 min和120 min的存活率、对大蜡螟Galleria mellonella末龄幼虫致死率和水平运动能力的差异。紫外胁迫后,不同介质中储存的海滨斯氏线虫的平均存活率从大到小依次为预胶化淀粉(69.39%)、木质素磺酸钙(51.75%)、蛭石(48.06%)、无菌蒸馏水(47.17%)、黑炭(16.92%)。储存于木质素磺酸钙中的海滨斯氏线虫经紫外胁迫30 min后处理大蜡螟幼虫，其72 h的校正致死率在93.33%~96.67%;紫外胁迫60 min后,储存于预胶化淀粉、蛭石、木质素磺酸钙中的海滨斯氏线虫对大蜡螟幼虫的校正致死率在83.33%~90.00%;紫外胁迫90 min后,储存于蛭石中海滨斯氏线虫对大蜡螟幼虫的校正致死率在90.00%~96.67%;紫外胁迫120 min后,5种介质中的海滨斯氏线虫对大蜡螟幼虫的校正致死率在60.00%~76.67%，无显著差异(P>0.05)。紫外胁迫4个处理中,储存于木质素磺酸钙中的海滨斯氏线虫24 h后92.33%~96.67%的个体运动到3.6 cm圆孔中。研究结果表明,与测定的其他介质相比，木质素磺酸钙能显著提高海滨斯氏线虫抗紫外胁迫的能力。因此,推荐使用木质素磺酸钙储存海滨斯氏线虫，以增强其对紫外线的耐受能力。
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| 关 键 词： | 紫外胁迫 不同介质 海滨斯氏线虫 生活力 生物防治 |
| 收稿时间： | 2020/1/13 0:00:00 |
| 修稿时间： | 2020/3/17 0:00:00 |
Effects of different media on the resistance of Steinernema litorale to ultraviolet radiation |
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| XIAO Jianfei,HAN Lanlan,ZHAO Kuijun,HAO Ziru,ZHU Lin,SHI Zhenghao,GAO Litong,CHEN Juan.Effects of different media on the resistance of Steinernema litorale to ultraviolet radiation[J].Plant Protection,2021,47(3):115-121. |
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| Authors: | XIAO Jianfei HAN Lanlan ZHAO Kuijun HAO Ziru ZHU Lin SHI Zhenghao GAO Litong CHEN Juan |
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| Institution: | College of Agriculture, Northeast Agricultural University, Harbin 150030, China |
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| Abstract: | In order to screen appropriate media to enhance the resistance of entomopathogenic nematodes to UV radiation,the Steinernema litorale stored in calcium lignosulfonate, pregelatinized starch, vermiculite, black carbon, and sterile distilled water (CK), were exposed to 18 W UV lamp at 30 cm for 30, 60, 90 and 120 minutes. The survival rates of S. litorale after UV irradiation, the mortalities of the last instar larvae of Galleria mellonella treated by survival S. litorale for 72 hours and the transverse diffusion ability of survival S. litorale were investigated. The results showed that after UV irradiation, the average survival rates of S. litorale was 69.39%, 51.75%, 48.06%, 47.17% and 16.92% for media of pregelatinized starch, calcium lignosulfonate, vermiculite, sterile distilled water and black carbon, respectively. The corrected mortalities of G. mellonella was 93.33%-96.67% when treated for 72 hours by S. litorale stored in calcium lignosulfonate and survived 30 min UV irradiation. The corrected mortality of G. mellonella treated by S. litorale for 72 hours were 83.33%-90.00% when S. litorale stored in calcium lignosulfonate, pregelatinized starch and vermiculite and exposed to UV radiation for 60 min, and the corrected mortalities of G. mellonella larvae were 90.00%-96.67% when S. litorale stored in vermiculite and exposed to UV radiation for 90 min. The corrected mortality of G. mellonella treated with S. litorale stored in five media and exposed to UV radiation for 120 min were 60.00%-76.67%, and showed no significant difference (P>0.05). There was 92.33%-96.67% individuals that stored in calcium lignosulfonate could move 3.6 cm to reach the circular hole in 24 hours in all treatments. The results showed that calcium lignosulfonate had the best protection for S. litorale in resistance to UV radiation. Therefore, the calcium lignosulfonate was recommended to store the S. litorale. |
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| Keywords: | ultraviolet stress different media Steinernema litorale life activity biological control |
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