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枇杷属植物核型分析及其在系统分类中的应用
引用本文:李桂芬,梁国鲁,林顺权.枇杷属植物核型分析及其在系统分类中的应用[J].园艺学报,2013,40(8):1465-1474.
作者姓名:李桂芬  梁国鲁  林顺权
作者单位:(1华南农业大学园艺学院,广州 510642;2广西壮族自治区农业科学院园艺研究所,南宁 530007;3西南大学园艺园林学院,重庆 400715)
基金项目:公益性行业科研专项,亚热带农业生物资源保护与利用国家重点实验室项目,广东普通高校重点实验室华南园艺作物种质资源创新与利用项目
摘    要: 对21个枇杷属材料和1个枇杷近缘属材料(石斑木)用去壁低渗-火焰干燥法进行染色体制片,进行核型分析,结果发现:所试材料的体细胞染色体数均为2n = 34,为二倍体,染色体类型包括中部(m)、近中部(sm)和近端部(st)着丝点染色体(st,仅存在于大花枇杷中)3种,核型公式为2n = 2x = 34 = 16 ~ 24m + 10 ~ 18sm(+ 2st),属2A对称型核型。根据各个种的中部着丝点染色体(m)的多少,可将22个材料初步分为5类,从第1类到第5类枇杷,核型不对称性依次增强,表明其系统演化地位逐渐进化。

关 键 词:枇杷属  核型分析  系统演化
收稿时间:2013-01-04

A Study on Karyotype Analysis of Genus Eriobotrya and Its Application to Systematic Taxonomy
LI Gui-fen,LIANG Guo-lu,LIN Shun-quan,.A Study on Karyotype Analysis of Genus Eriobotrya and Its Application to Systematic Taxonomy[J].Acta Horticulturae Sinica,2013,40(8):1465-1474.
Authors:LI Gui-fen    LIANG Guo-lu  LIN Shun-quan  
Institution:(1 College of Horticulture,South China Agricultural University,Guangzhou 510642,China;2 Horticulture Research Institute,Guangxi Academy of Agriculture Sciences,Nanning 530007,China;3College of Horticulture and Lanscape Architecture,Southwest University,Chongqing 400715,China)
Abstract:In this paper the wall degradation hypotonic method was used to prepare mitotic chromosome sample of 21 materials(16 species,4 varieties,1 interspecific hybrid)of Eriobotrya and 1 closed genus of EriobotryaRhaphiolepis indica(L.)Lindl.]. The results indicated that the chromosome number was 2n = 2x = 34 in all the materials,and the karyotypes of Eriobotrya and Rhaphiolepis indica consist of three kinds of chromosome:m,sm,and st(only in E. cavaleriei Rehd). The karyotype formula was 2n = 2x = 34 =(16 to 24)m +(10 to 18)sm(+ 2st)for all materials. According to the karyotype data,it can be concluded that the karyotypes of 21 Eriobotrya materials belongs to Stebbins’2A,which is a symmetrical karyotype. According to the karyotype formula we preliminarily classified these Eriobotrya materials into 5 groups. The asymmetry increased gradually from the first group to the fifth group,which indicated the systematic evolution among groups is more and more advanced.
Keywords:Eriobotrya  karyotype analysis  evolution
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