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Neutrophil adherence,phagocytic-nitroblue tetrazolium reduction and chemiluminescence in canine whole blood |
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| Authors: | H Nagahata T Sako J A Reiter S P Dibartola C G Couto G J Kociba |
| Institution: | (1) Department of Veterinary Pathobiology, College of Veterinary Medicine, The Ohio State University, Coffey Road 1925, 43210 Columbus, OH, USA;(2) Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Coffey Road 1925, 43210 Columbus, OH, USA;(3) Present address: Department of Animal Health, School of Veterinary Medicine, Rakuno Gakuen University, 069 Ebetsu, Hokkaido, Japan |
| Abstract: | Methods for measuring neutrophil adherence, phagocytic-nitroblue tetrazolium (NBT) reducing activity and chemiluminescence were applied to canine whole blood as means for routine assessment of neutrophil functions. The phagocytic-NBT reduction test appeared to be useful for monitoring the NBT reducing activity of phagocytic cells associated with phagocytic functions. Ethylene diamine tetraacetic acid suppressed both the adherence and the phagocytic-NBT reducing activity of neutrophils. Increased phagocytic-NBT reduction and an enhanced chemiluminescence response were observed in dogs with neutrophilia. These methods provide a rapid and practical screening procedure for measuring selected phagocytic functions in canine whole blood. |
| Keywords: | adherence assay chemiluminescence dog screen neutrophils tetrazolium |
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