| 桃PGIP基因及cDNA的克隆及序列分析 |
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| 引用本文： | 谌悦,张军科,熊帅.桃PGIP基因及cDNA的克隆及序列分析[J].安徽农业科学,2009,37(32):15738-15740. |
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| 作者姓名： | 谌悦 张军科 熊帅 |
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| 作者单位： | 西北农林科技大学园艺学院,农业部西北园艺植物种质资源利用重点开放实验室,陕西杨凌,712100;西北农林科技大学园艺学院,农业部西北园艺植物种质资源利用重点开放实验室,陕西杨凌,712100;西北农林科技大学园艺学院,农业部西北园艺植物种质资源利用重点开放实验室,陕西杨凌,712100 |
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| 摘 要： | 通过PCR扩增了桃的PGIP基因及eDNA序列，并进行了序列之间的比对及分析。结果表明，桃的PGIP基因全长1092bp，而其cDNA序列只有1045bp，PGIP基因中含有一段长147bp的内含子，且内含子符合GT-AG规律。这与其他核果类植物的PGIP基因构成基本相同。其基因序列与GenBank中已登录的3个桃以及其他核果的序列比对，其同源性达92％～99％；与苹果、梨和大桉的同源性分别为85％、84％和8．4％。
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| 关 键 词： | 桃 PGIP基因 克隆 序列分析 |
Cloning and Sequence Analysis of PGIP gene and cDNA from Prunus persica |
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| CHEN Yue et al.Cloning and Sequence Analysis of PGIP gene and cDNA from Prunus persica[J].Journal of Anhui Agricultural Sciences,2009,37(32):15738-15740. |
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| Authors: | CHEN Yue |
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| Institution: | CHEN Yue et al (College of Horticulture,Northwest A & F University,Key Lab of Northwestern Horticultural plant Germplasm Resource Application of Ministry of Agriculture,Yangling,Shaanxi 712100) |
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| Abstract: | PGIP gene and cDNA were cloned from Prunus persica and the sequences were compared and analyzed.The result showed that the length of PGIP gene was 1 092 bp,while the length of cDNA was only 1 045 bp.The PGIP gene contained 147 bp length intron,and according to GT-AG law,which basically the same with PGIP gene of other stone fruit plants.its gene sequence and logged 3 peaches in GenBank and other stone fruit were compared the homology can reached to 92%-99%.The homology rates of PGIPs,compared peach with Mal... |
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| Keywords: | Prunus persica PGIP Clone Sequence analysis |
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