| 湖羊高繁殖力候选基因ESR的研究 |
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| 引用本文： | 董文艳,陈阿琴,王争光,俞颂东.湖羊高繁殖力候选基因ESR的研究[J].浙江农业学报,2009,21(6):0. |
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| 作者姓名： | 董文艳 陈阿琴 王争光 俞颂东 |
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| 作者单位： | 1.浙江大学 动物科学学院，浙江 杭州 310029；;2.上海海洋大学 水产与生命学院，上海201306 |
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| 基金项目： | 浙江省科技厅重大项目，中国博士后科学基金资助项目，浙江省教育厅科研项目 |
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| 摘 要： | 利用 PCR-SSCP技术对高繁殖力绵羊品种湖羊雌激素受体（estrogen receptor，ESR）基因第一外显子的部分序列进行单核苷酸多态性研究。结果表明，湖羊ESR基因中存在 3 种基因型 AA，BB，AB，且A等位基因频率为0.554，B 等位基因频率为0.446。经测序，BB型与AA型相比在外显子 1 第 363 位发生 1 处C→G的碱基突变。据产羔数统计，AB 基因型和 BB 基因型的湖羊产羔数分别比 AA 基因型多0.98只（P<0.01）、1.47只（P<0.01）。说明ESR基因可能是控制湖羊多羔性能的一个主效基因或与之存在紧密遗传连锁的一个标记。
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| 关 键 词： | 湖羊 高繁殖力 候选基因 雌激素受体基因 PCR-SSCP |
Estrogen receptor as a candidate gene for prolificacy of Hu sheep |
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| DONG Wen-yan,CHEN A-qin,WANG Zheng-guang,YU Song-dong.Estrogen receptor as a candidate gene for prolificacy of Hu sheep[J].Acta Agriculturae Zhejiangensis,2009,21(6):0. |
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| Authors: | DONG Wen-yan CHEN A-qin WANG Zheng-guang YU Song-dong |
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| Institution: | 1 College of Animal Science，Zhejiang University，Hangzhou 310029，China；2 College of Fisheries and Life Science， Shanghai Ocean University，Shanghai 201306，China |
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| Abstract: | Single nucleotide polymorphism in exon 1 of the estrogen receptor (ESR) gene was detected by PCR-SSCP in high fecundity Hu sheep breed. The results indicated that there were three genotypes (AA,BB,AB) in Hu sheep and the frequency of allele A and B was 0.554 and 0.446,respectively. Comparison with the AA genotype, sequencing revealed a C→G mutation at 363 bp of exon 1 of ESR gene in the BB genotype. The Hu sheep with AB genotype or BB genotype had 0.98 and 1.47 more Iambs than those with AA genotype,respectively. These results showed that the estrogen receptor locus was a major gene that controlled the prolificacy in Hu sheep or a mark in close linkage with such a gene. |
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| Keywords: | PCR-SSCP Hu sheep (Ovis aries) prolificacy candidate gene estrogen receptor gene PCR-SSCP |
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