| 高致病性禽流感RT-PCR快速诊断方法的建立 |
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| 引用本文： | 彭晓玲,兰玲,马亚珍,成进.高致病性禽流感RT-PCR快速诊断方法的建立[J].动物医学进展,2009,30(1). |
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| 作者姓名： | 彭晓玲 兰玲 马亚珍 成进 |
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| 作者单位： | 1. 新疆自治区动物卫生监督所,新疆乌鲁木齐,830063
2. 新疆自治区畜牧科学院,新疆乌鲁木齐,830000 |
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| 摘 要： | 参照国内外方法,选择M基因的保守序列作为靶序列,应用快速、高效、特异的一步法RT-PCR检测法进行禽流感病毒型特异性诊断,得到大小为229bp的特异性产物,与预期结果一致.另外,采用柱式总RNA提取方法,与酚/氯仿的抽提方法相比,具有快速,对操作人员技术要求较低,整个反应和扩增体系设计合理等特点.以上技术,简化了操作程序,节约了检测时间,缩小了因微量操作引起的误差,可获得较理想的结果和可靠的检测数据.
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| 关 键 词： | 禽流感病毒 一步法 |
Establishment of RT-PCR for Rapid Diagnosis of Highly Pathogenic avian influenza |
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| PENG Xiao-ling,LAN Ling,MA Ya-zhen,CHENG Jin.Establishment of RT-PCR for Rapid Diagnosis of Highly Pathogenic avian influenza[J].Progress In Veterinary Medicine,2009,30(1). |
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| Authors: | PENG Xiao-ling LAN Ling MA Ya-zhen CHENG Jin |
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| Institution: | 1.Institute of Animal Sanitary Supervision of Xinjiang Uygur Autonomous Region;Urumqi;Xinjiang;830063;China;2.Xinjiang Autonomous Region Academy of Animal Husbandry;830000;China |
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| Abstract: | According to the PCR technology based on the latest research development at home and abroad,choosing the M gene conservative sequence as a target sequence,the fast,efficient,specific one-step RT-PCR method was used for detecting avian influenza virus.A 229 bp specific product was obtained,which is consistent with the anticipated result.In addition,the column method was used for total RNA extraction.Compared with the phenol/chloroform extraction method,the column method was fast and simple,and the entire amp... |
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| Keywords: | RT-PCR |
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