| 楸树组培初代培养技术 |
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| 引用本文： | 傅玉兰,费鹏飞,刘小云.楸树组培初代培养技术[J].林业科技开发,2009,23(4):88-91. |
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| 作者姓名： | 傅玉兰 费鹏飞 刘小云 |
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| 作者单位： | 安徽农业大学林学与园林学院,合肥,230036 |
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| 摘 要： | 以楸树幼嫩茎段为试验材料,对组培初代培养阶段外植体无菌体系的建立和腋芽诱导影响因素进行了研究探讨.结果表明:茎段外植体最佳灭菌措施为:自来水冲洗30 min→70%酒精浸泡6 s→无菌水冲洗2次→(100mg/L青霉素+100 mg/L链霉素)浸20 min→0.1%升汞液浸泡10 min→无菌水冲洗8次→100 mg/L PVP浸泡5min.楸树茎段腋芽诱导的最适宜培养基为:1/2MS+BA1.0 mg/L+ZT 0.2 mg/L+IBA0.2 mg/L.添加食糖30 g/L琼脂8g/L,调整pH5.8.
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| 关 键 词： | 楸树 初代培养 腋芽诱导 |
Technique of Inducing in Vitro Culture of Catalpa bungei C.A. Mey |
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| FU Yu-lan,FEI Peng-fei,LIU Xiao-yun.Technique of Inducing in Vitro Culture of Catalpa bungei C.A. Mey[J].China Forestry Science and Technology,2009,23(4):88-91. |
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| Authors: | FU Yu-lan FEI Peng-fei LIU Xiao-yun |
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| Institution: | FU Yu-lan,FEI Peng-fei,LIU Xiao-yun College of Forestry & Gardens,Anhui Agruicultural University,230036 Hefei,China |
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| Abstract: | |
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| Keywords: | Catalpa bungei C A Mey Inducing culture Axillary bud induction |
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