Comparing RAPD and AFLPTM analysis in discrimination and estimation of genetic similarities among apple (Malus domestica Borkh.) cultivars |
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| Authors: | Luís Goulão Luís Cabrita Cristina M Oliveira José M Leitão |
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| Institution: | (1) Secção de Horticultura – Instituto Superior de Agronomia – Tapada da Ajuda, 1349-018 Lisboa, Portugal;(2) UCTA – Universidade do Algarve, Campus de Gambelas, 8000 Faro, Portugal |
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| Abstract: | Forty-one apple (Malus × domestica Borkh.) cultivars were screened for RAPD (Random Amplified Polymorphic DNA) and AFLP(Amplified Fragment Length Polymorphism)
markers. RAPD analysis was performed with 35 arbitrary 10-mer primers, selected from 60 primers tested (kits A, C and E, Operon
Technologies, Inc.). Of a total of 362bands observed, 208 (57.5%) were polymorphic. Three-hundred-and-eighty-one AFLP fragments
were obtained with 8primer combinations, of which 218 (57.2%) were polymorphic. Cultivars differentiated through mutation
were included in this study and showed identical patterns when analysed with both RAPD and AFLP analysis. The estimated genetic
relationships were correlated (r = 73.7%) between the analysis with the two different markers. UPGMA analysis was performed
and dendrograms were constructed using either the data apart from each(RAPD and AFLP) method or combined in a single joint
matrix. The relationships among the forty-one studied cultivars were basically consistent with the known lineage and geographic
origins of the cultivars. The four Portuguese cultivars included in this study clustered together and diverged from the other
cultivars. Apparently they constitute an independent genetic pool, which could be of interest for apple plant breeders.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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| Keywords: | AFLP apples genetic similarity molecular markers RAPD |
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