| 籼粳交DH群体和RIL群体的构建及籼粳分化 |
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| 引用本文： | 程式华,毛传澡,占小登,斯华敏,孙宗修.籼粳交DH群体和RIL群体的构建及籼粳分化[J].中国水稻科学,2001,15(4):257-260. |
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| 作者姓名： | 程式华 毛传澡 占小登 斯华敏 孙宗修 |
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| 作者单位： | 中国水稻研究所国家水稻改良中心 |
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| 基金项目： | 国家自然科学基金资助项目(39970448);浙江省青年科技人才培养专项基金资助项目(RC97-20). |
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| 摘 要： | 对籼粳杂交组合“02428（粳）/特青（籼）”F1进行一步成苗法花药培养，对该组合的分离世代采用连续自交随机选择，分别获得由132个株系组成的加倍单倍体（DH）群体和由157个株系组成的重组自交系（RIL）群体。通过形态指数法和分子标记法对两个群体的籼粳分化进行了研究，结果表明，无论是DH群体还是RIL群体，各株系的籼粳形态指数和分子标记指数分布均呈正态分布，但RIL群体的偏态性强于DH群体，且明显偏父。
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| 关 键 词： | 加倍单倍体 重组自交系 |
| 文章编号： | 1001-7216(2001)04-0257-04 |
| 收稿时间： | 1900-01-01; |
Construction of Double Haploid (DH) and Recombinant Inbred Line (RIL) Population of Indica-Japonica Hybrid and Their Differential in Indica and Japonica Property |
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| CHENG Shi hua ,MAO Chuan zao ,ZHAN Xiao deng ,SI Hua min ,SUN Zong xiu.Construction of Double Haploid (DH) and Recombinant Inbred Line (RIL) Population of Indica-Japonica Hybrid and Their Differential in Indica and Japonica Property[J].Chinese Journal of Rice Science,2001,15(4):257-260. |
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| Authors: | CHENG Shi hua MAO Chuan zao ZHAN Xiao deng SI Hua min SUN Zong xiu |
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| Institution: | CHENG Shi hua 1,2,MAO Chuan zao 1,ZHAN Xiao deng 1,SI Hua min 2,SUN Zong xiu 2 |
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| Abstract: | A double haploid (DH) population with 132 plant lines and a recombination inbred lines (RIL) with 152 plant lines were constructed through one step anther culture and continuous self pollination of the hybrid between 02428 (japonica) and Teqing (indica), respectively. The differentiation of indica and japonica in the two populations was detected by the morphological index and RFLP method. All the morphological traits of DH and RIL populations were in continuous segregation and showed normal distribution. It was indicated that the segregation and recombination of genes of indica and japonica in DH population were basically random and the overall frequency of alleles did not deviate from the ratio of 1∶1. But RIL population was significantly tended to the indica parent. |
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| Keywords: | double haploid recombination inbred line differentiation of indica and japonica |
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