| 水稻纹枯病菌粗毒素提取及活性初步研究 |
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| 引用本文： | 黄文文,向准,龚亮,钟国华.水稻纹枯病菌粗毒素提取及活性初步研究[J].安徽农业科学,2009,37(27):13342-13345. |
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| 作者姓名： | 黄文文 向准 龚亮 钟国华 |
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| 作者单位： | 华南农业大学昆虫毒理研究室,广东广州,510642 |
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| 基金项目： | 国家自然科学基金，教育部全国优秀博士学位论文作者专项基金项目 |
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| 摘 要： | 目的]研究水稻纹枯病菌的致病机制及毒素的活性成分。方法]将水稻纹枯病菌Rhizoctoniasolani在改良Richard培养基中培养，培养滤液经离心、灭菌、活性炭粒吸附层析、甲醇洗脱、蒸馏水洗脱、浓缩得培养液粗提物、甲醇粗提物以及水粗提物，并对3种粗提物进行活性测定。结果]3种粗提物均为黄褐色膏状物；活性测定表明，3种粗提物对水稻胚根、胚芽都有明显的生长抑制作用，对水稻4叶期幼苗都有致萎作用，对水稻离体叶片也有不同程度的毒害作用，能产生与病原茵侵染类似的云纹状褪绿斑。结论]水稻纹枯病茵毒素的活性组分可能有多种成分。
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| 关 键 词： | 水稻纹枯病 粗毒素 提取 生物活性 |
Preliminary Study on the Extraction of Crude Toxin of Rhizoctonia solani and Its Activity |
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| HUANG Wen-wen et al.Preliminary Study on the Extraction of Crude Toxin of Rhizoctonia solani and Its Activity[J].Journal of Anhui Agricultural Sciences,2009,37(27):13342-13345. |
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| Authors: | HUANG Wen-wen |
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| Institution: | HUANG Wen-wen et al(Laboratory of Insect Toxicology,South China Agricultural University,Guangzhou,Guangdong 510642) |
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| Abstract: | Objective] This study was to investigate the pathogenic mechanism of rice sheath blight pathogen(Rhizoctonia solani) and the bioactive components of toxin.Method] Rice sheath blight pathogen was cultured in the improved Richard medium;the culture filtrates were centrifuged and sterilized,then treated by activated carbon adsorption chromatography,distilled with methanol or water,and all were next concentrated,yielding the crude extracts of culture solution,crude extracts of methanol and crude extracts of w... |
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| Keywords: | Rice sheath blight Crude toxin Extraction Bioactivity |
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