Production and characterization of intergeneric diploid cybrids derived from symmetric fusion between Microcitrus papuana Swingle and sour orange (Citrus aurantium) |
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| Authors: | Xiaoyong Xu Jihong Liu Xiuxin Deng |
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| Institution: | (1) National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, Wuhan, 430070, P.R. China |
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| Abstract: | Plants were regenerated from intergeneric somatic hybridization between embryogenic protoplasts of Microcitrus papuana Swingle and leaf-derived protoplasts of sour orange (Citrus aurantium L.) via electrofusion. The regenerated plants were morphologically similar to the leaf parent in growth vigor, leaf and branch
structure. FCM analysis showed that they were diploids. Simple-sequence-repeat (SSR) and cleaved-amplified-polymorphic-sequence(CAPS)
were employed for hybridity characterization. SSR banding patterns of the regenerated plants were identical to the leaf parent,
sour orange, indicating that they possessed nuclear component derived from sour orange. DNA amplification with chloroplast
and mitochondrial universal primers, followed by restriction endonuclease digestion, revealed polymorphism between the fusion
parents. Therefore, this method was used to determine the cytoplasmic compositions of the regenerated plants. Banding patterns
for all the polymorphic primer/enzyme combinations of the regenerated plants were similar to those of the embryogenic parent,
M. papuana, suggesting that only the cytoplasmic components derived from the embryogenic parent were present in the regenerated plants.
FCM, SSR and CAPS demonstrated that intergeneric diploid cybrids have been successfully obtained by symmetric fusion. Related
results concerning nuclear and cytoplasmic composition of previous diploid somatic hybrids and potential mechanism for regeneration
of such kind of plants are discussed herein.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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| Keywords: | CAPS Citrus cpDNA cybrids flow cytometry Microcitrus mtDNA SSR |
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